Overview
- External ontology
- mediadive.medium:1335 (source link unavailable) — MODIFIED T5 MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1335.pdf
Composition
flowchart LR
CultureMech_000792["modified_t5_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_000792 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000792 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_000792 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Starch["Starch"]
CultureMech_000792 --> ing3_Starch
CHEBI_28017["CHEBI:28017"]
ing3_Starch -.-> CHEBI_28017
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000792 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Agar["Agar"]
CultureMech_000792 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 0.5 G_PER_L | ||
| 2 | Yeast extract | 2 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust to pH 7.0-7.5 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_t5_medium (CultureMech:000792)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.129645Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1335 |
| 2026-03-13T06:18:58.395092Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000792 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.798782+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED T5 MEDIUM" to "modified_t5_medium" for consistent matching |
| 2026-05-20T06:01:59.785170+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.5' -> {'min': 7.0, 'max': 7.5} |
| 2026-06-05T07:41:37.862119+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:49.893741+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 11257e8b817c1893f4b405ac1bde5c4f062d4cef94b9726db29254405e66c7e4 |