methylophaga_medium

CultureMech:000794 bacterial DEFINED SOLID_AGAR
Original name: METHYLOPHAGA MEDIUM

Overview

External ontology
mediadive.medium:1337 (source link unavailable) — METHYLOPHAGA MEDIUM
Applications
  • Microbial cultivation
pH
8.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1337.pdf

Composition

flowchart LR
    CultureMech_000794["methylophaga_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000794 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000794 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000794 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_000794 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2["CaCl2"]
    CultureMech_000794 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_Agar["Agar"]
    CultureMech_000794 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Methanol["Methanol"]
    CultureMech_000794 --> ing6_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing6_Methanol -.-> CHEBI_17790
    ing7_KH2PO4["KH2PO4"]
    CultureMech_000794 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_NH4Cl["NH4Cl"]
    CultureMech_000794 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_Bis_Tris["Bis-Tris"]
    CultureMech_000794 --> ing9_Bis_Tris
    CHEBI_41250["CHEBI:41250"]
    ing9_Bis_Tris -.-> CHEBI_41250
    ing10_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_000794 --> ing10_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing10_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000794 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MgSO4["MgSO4"]
    CultureMech_000794 --> ing12_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing12_MgSO4 -.-> CHEBI_32599
    ing13_MnSO4["MnSO4"]
    CultureMech_000794 --> ing13_MnSO4
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4 -.-> CHEBI_86360
    ing14_FeSO4["FeSO4"]
    CultureMech_000794 --> ing14_FeSO4
    CHEBI_75832["CHEBI:75832"]
    ing14_FeSO4 -.-> CHEBI_75832
    ing15_CoCl2["CoCl2"]
    CultureMech_000794 --> ing15_CoCl2
    CHEBI_35696["CHEBI:35696"]
    ing15_CoCl2 -.-> CHEBI_35696
    ing16_ZnSO4["ZnSO4"]
    CultureMech_000794 --> ing16_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing16_ZnSO4 -.-> CHEBI_35176
    ing17_CuSO4["CuSO4"]
    CultureMech_000794 --> ing17_CuSO4
    CHEBI_23414["CHEBI:23414"]
    ing17_CuSO4 -.-> CHEBI_23414
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_000794 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_000794 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4["Na2MoO4"]
    CultureMech_000794 --> ing20_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing20_Na2MoO4 -.-> CHEBI_75215
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_000794 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
25.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 24.0, 1.0]
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
5 CaCl2
Ca.2Cl 110.984 g/mol
1.1 G_PER_L CHEBI:3312
calcium dichloride
 [Merged 2 duplicates: 1.0, 0.1]
6 Agar 20 G_PER_L CHEBI:2509
agar
 (for solid medium)
7 Methanol
CH4O 32.042 g/mol
2.376 G_PER_L CHEBI:17790
methanol
8 KH2PO4
H2O4P.K 136.084 g/mol
7 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 NH4Cl
Cl.H4N 53.492 g/mol
100 G_PER_L CHEBI:31206
ammonium chloride
10 Bis-Tris
C8H19NO5 209.242 g/mol
100 G_PER_L CHEBI:41250
bis-tris
11 Ferric ammonium citrate 3 G_PER_L CHEBI:31604
ferric ammonium citrate
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MgSO4
Mg.O4S 120.368 g/mol
3 G_PER_L CHEBI:32599
magnesium sulfate
14 MnSO4
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
15 FeSO4
Fe.O4S 151.908 g/mol
0.1 G_PER_L CHEBI:75832
iron(2+) sulfate (anhydrous)
16 CoCl2
2Cl.Co 129.839 g/mol
0.1 G_PER_L CHEBI:35696
cobalt dichloride
17 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
18 CuSO4
Cu.O4S 159.609 g/mol
0.01 G_PER_L CHEBI:23414
copper(II) sulfate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4
MoO4.2Na 205.916 g/mol
0.01 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
22 Vitamin B12 0.1 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Adjust to pH 8.0 and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
  2. After autoclaving cool down and add the following to 1 litre medium. Methanol and Vitamine B12 are filter sterilized. Solution T and Wolfe's Mineral Solution are separately autoclaved. Final pH 8.0.
  3. Adjust to pH 8.0 and sterilize by autoclaving.
  4. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals.
  5. Adjust to pH 8.0 with KOH and sterilize by autoclaving.
  6. Filter sterilized

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.133111Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1337
2026-03-13T03:08:55.827649Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:58.407959Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000794
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.813597+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOPHAGA MEDIUM" to "methylophaga_medium" for consistent matching
2026-06-05T07:41:33.283997+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.618797+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:45.000699+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 79ad319410839fe99c04e2ab14423a964f397c462e27d49e342dfdbcdd8901ec