Overview
- External ontology
- mediadive.medium:1338 (source link unavailable) — LB MEDIUM WITH CARBONATES
- Applications
-
- Microbial cultivation
- pH
- 9.6
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1338.pdf
Composition
flowchart LR
CultureMech_000795["lb_medium_with_carbonates"]
ing0_NaHCO3["NaHCO3"]
CultureMech_000795 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Na2CO3["Na2CO3"]
CultureMech_000795 --> ing1_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing1_Na2CO3 -.-> CHEBI_29377
ing2_Tryptone["Tryptone"]
CultureMech_000795 --> ing2_Tryptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_000795 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_000795 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Agar["Agar"]
CultureMech_000795 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 2 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 3 | Tryptone | 20 G_PER_L | ||
| 4 | Yeast extract | 10 G_PER_L | ||
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 | Agar | 40 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Autoclave the Mineral Salts and the Carbonate Salts separately. Combine both solutions after cooling. The medium may be solidified by adding 20.0 g/l agar. The agar is added to Minerals Salts Solution and after autoclaving both the Mineral Salts Solution and the Carbonate Salts Solution are cooled to 50-55°C, mixed and poured into Petri dishes. Final pH 9.6.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lb_medium_with_carbonates (CultureMech:000795)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.137112Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1338 |
| 2026-03-13T06:18:58.414325Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000795 |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.821060+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB MEDIUM WITH CARBONATES" to "lb_medium_with_carbonates" for consistent matching |
| 2026-06-05T07:41:10.927736+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.493139+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:40.062983+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 08c3d6fff9218adc11419205536059975b364834fe3131b4026ca687219621fb |