m2_medium

CultureMech:000805 bacterial DEFINED LIQUID
Original name: M2-MEDIUM

Overview

External ontology
mediadive.medium:1347 (source link unavailable) — M2-MEDIUM
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1347.pdf

Composition

flowchart LR
    CultureMech_000805["m2_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_000805 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000805 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000805 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000805 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NaCl["NaCl"]
    CultureMech_000805 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_EDTA["EDTA"]
    CultureMech_000805 --> ing5_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing5_EDTA -.-> CHEBI_4735
    ing6_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_000805 --> ing6_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing6_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000805 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000805 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000805 --> ing9_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000805 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_Na2MoO4["Na2MoO4"]
    CultureMech_000805 --> ing11_Na2MoO4
    CHEBI_75215["CHEBI:75215"]
    ing11_Na2MoO4 -.-> CHEBI_75215
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000805 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.24975 G_PER_L CHEBI:63043
potassium nitrate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.0999001 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.04995 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.00999001 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NaCl
Cl.Na 58.443 g/mol
0.01998 G_PER_L CHEBI:26710
sodium chloride
6 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
7 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.1 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 Na2MoO4
MoO4.2Na 205.916 g/mol
0.03 G_PER_L CHEBI:75215
sodium molybdate (anhydrous)
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate

Preparation

  1. Prepare the liquid medium. Final pH 5.5.The medium is fairly weakly buffered so the pH should be checked before and after autoclaving. Dispense the medium into serum bottles. Add 10-20% methane to the gas phase. Sterilize by autoclaving.
  2. The strain should be grown with shaking.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.159810Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1347
2026-03-13T06:18:58.488493Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000805
2026-03-17T06:29:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:09.908732+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M2-MEDIUM" to "m2_medium" for consistent matching
2026-06-05T07:41:11.796591+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.058744+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:00.681569+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:40.861400+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint e129f7d67c1a23c5c6e8585a6935dbf63fde825569aa1d5a718363c8b82d8dee