Overview
- External ontology
- mediadive.medium:1356 (source link unavailable) — HMD MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1356.pdf
Composition
flowchart LR
CultureMech_000814["hmd_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000814 --> ing0_Yeast_extract
ing1_Casamino_acids["Casamino acids"]
CultureMech_000814 --> ing1_Casamino_acids
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000814 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000814 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_K2SO4["K2SO4"]
CultureMech_000814 --> ing4_K2SO4
CHEBI_32036["CHEBI:32036"]
ing4_K2SO4 -.-> CHEBI_32036
ing5_NH4Cl["NH4Cl"]
CultureMech_000814 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_KH2PO4["KH2PO4"]
CultureMech_000814 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NaCl["NaCl"]
CultureMech_000814 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_Agar["Agar"]
CultureMech_000814 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.1 G_PER_L | ||
| 2 | Casamino acids | 0.5 G_PER_L | ||
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 | Agar | 12 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust to pH 7.5 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hmd_medium (CultureMech:000814)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.182770Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1356 |
| 2026-01-28T19:48:12.974130Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.553230Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000814 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:09.980733+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HMD MEDIUM" to "hmd_medium" for consistent matching |
| 2026-06-05T07:41:08.609474+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.833135+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:59.794650+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.693525+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4e66a801597ff14e037021cfdd5217b94c9c0557fb32d011b07a33e1d6ef2a57 |