methylovirgula_medium

CultureMech:000828 bacterial DEFINED LIQUID
Original name: METHYLOVIRGULA MEDIUM

Overview

External ontology
mediadive.medium:1368 (source link unavailable) — METHYLOVIRGULA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 5.0, 'max': 5.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1368.pdf

Composition

flowchart LR
    CultureMech_000828["methylovirgula_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_000828 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000828 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000828 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000828 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Methanol["Methanol"]
    CultureMech_000828 --> ing4_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing4_Methanol -.-> CHEBI_17790

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.15 G_PER_L CHEBI:63043
potassium nitrate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 Methanol
CH4O 32.042 g/mol
0.1 G_PER_L CHEBI:17790
methanol

Preparation

  1. Prepare the liquid medium. Adjust to pH 5.0 - 5.5. Dispense the medium into Hungate tubes. Sterilize by autoclaving. Before inoculation add filter sterilized methanol (0.1% v/v).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.219830Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1368
2026-03-13T06:18:58.692824Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000828
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.135530+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOVIRGULA MEDIUM" to "methylovirgula_medium" for consistent matching
2026-05-20T06:01:57.086524+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5-5.5' -> {'min': 5.0, 'max': 5.5}
2026-06-05T07:41:33.557348+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.585047+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.725823+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:45.211299+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 11be53da07a1a2256aafdf417de84a099739e4180e33181560d3b27e1c1ae1f8