Overview
- External ontology
- mediadive.medium:1370 (source link unavailable) — RICH ORGANIC MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1370.pdf
Composition
flowchart LR
CultureMech_000831["rich_organic_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000831 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_KH2PO4["KH2PO4"]
CultureMech_000831 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_000831 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000831 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Na_acetate["Na-acetate"]
CultureMech_000831 --> ing4_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Na_acetate -.-> CHEBI_32954
ing5_Yeast_extract["Yeast extract"]
CultureMech_000831 --> ing5_Yeast_extract
ing6_Peptone["Peptone"]
CultureMech_000831 --> ing6_Peptone
ing7_Casamino_acids["Casamino acids"]
CultureMech_000831 --> ing7_Casamino_acids
ing8_Nicotinic_acid["Nicotinic acid"]
CultureMech_000831 --> ing8_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing8_Nicotinic_acid -.-> CHEBI_15940
ing9_Thiamine["Thiamine"]
CultureMech_000831 --> ing9_Thiamine
CHEBI_18385["CHEBI:18385"]
ing9_Thiamine -.-> CHEBI_18385
ing10_Biotin["Biotin"]
CultureMech_000831 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Vitamin_B12["Vitamin B12"]
CultureMech_000831 --> ing11_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing11_Vitamin_B12 -.-> CHEBI_176843
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000831 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000831 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000831 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_H3BO3["H3BO3"]
CultureMech_000831 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000831 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000831 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000831 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000831 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 6 | Yeast extract | 0.5 G_PER_L | ||
| 7 | Peptone | 0.5 G_PER_L | ||
| 8 | Casamino acids | 0.5 G_PER_L | ||
| 9 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.4 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 10 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.4 G_PER_L |
CHEBI:18385
thiamine(1+)
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.08 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 | Vitamin B12 | 0.02 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.3 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.005 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.003 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.002 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.005 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.001 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.002 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.003 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 5.9. Add NaOH (0.1 mol/l) 16ml/l after sterilisation from sterile stock solution to readjust pH to 6.8.
- Add the following from sterile stock solutions to the autoclaved medium:
- Filter sterilised
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rich_organic_medium (CultureMech:000831)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.224712Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1370 |
| 2026-01-28T19:48:13.195379Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.714773Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000831 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.160274+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RICH ORGANIC MEDIUM" to "rich_organic_medium" for consistent matching |
| 2026-06-05T07:41:44.559729+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:50.225199+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:11.043247+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.785132+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 075c9a2feb5fd53e2eaafcbb7548043f72c741e03afce1c46f26176bbcf20e02 |