ird_medium

CultureMech:000834 bacterial COMPLEX LIQUID
Original name: IRD MEDIUM

Overview

External ontology
mediadive.medium:1373 (source link unavailable) — IRD MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1373.pdf

Composition

flowchart LR
    CultureMech_000834["ird_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000834 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000834 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NaCl["NaCl"]
    CultureMech_000834 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_000834 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000834 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000834 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KCl["KCl"]
    CultureMech_000834 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000834 --> ing7_Yeast_extract
    ing8_Tryptone["Tryptone"]
    CultureMech_000834 --> ing8_Tryptone
    ing9_Cysteine_HCl["Cysteine HCl"]
    CultureMech_000834 --> ing9_Cysteine_HCl
    ing10_Resazurin["Resazurin"]
    CultureMech_000834 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_NaHCO3["NaHCO3"]
    CultureMech_000834 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_Na2S["Na2S"]
    CultureMech_000834 --> ing12_Na2S
    CHEBI_76208["CHEBI:76208"]
    ing12_Na2S -.-> CHEBI_76208
    ing13_Glucose["Glucose"]
    CultureMech_000834 --> ing13_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing13_Glucose -.-> CHEBI_17234
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000834 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000834 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000834 --> ing16_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing16_MnSO4_x_H2O -.-> CHEBI_86364
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000834 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000834 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000834 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000834 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000834 --> ing21_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing21_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing22_H3BO3["H3BO3"]
    CultureMech_000834 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000834 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000834 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000834 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NaCl
Cl.Na 58.443 g/mol
2.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 1.0, 1.0]
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.5 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.2 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.1, 0.1]
7 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
8 Yeast extract 1 G_PER_L
9 Tryptone 1 G_PER_L
10 Cysteine HCl 0.5 G_PER_L
11 Resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
12 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
13 Na2S
2Na.S 78.047 g/mol
0.2 G_PER_L CHEBI:76208
sodium sulfide (anhydrous)
14 Glucose
C6H12O6 180.156 g/mol
1.8016 G_PER_L CHEBI:17234
glucose
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
19 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
21 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
22 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
23 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Autoclave and add the following components after autoclaving from sterile stock solutions:
  2. pH 7.2
  3. Prepare the medium under a N2/CO2 atmosphere.
  4. The final pH of the medium should be 7.2.
  5. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.232604Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1373
2026-03-13T03:09:28.315228Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:58.738881Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000834
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.186762+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "IRD MEDIUM" to "ird_medium" for consistent matching
2026-06-05T07:41:09.573850+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.114768+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:38.635012+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f11cabe534ea05ff7fec37217762aeb87437740c524c9b972d7728ef9957169b