methylococcus_medium

CultureMech:000837 bacterial DEFINED LIQUID
Original name: METHYLOCOCCUS MEDIUM

Overview

External ontology
mediadive.medium:1376 (source link unavailable) — METHYLOCOCCUS MEDIUM
Applications
  • Microbial cultivation
pH
6.7
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1376.pdf

Composition

flowchart LR
    CultureMech_000837["methylococcus_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_000837 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000837 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000837 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KBr["KBr"]
    CultureMech_000837 --> ing3_KBr
    CHEBI_32030["CHEBI:32030"]
    ing3_KBr -.-> CHEBI_32030
    ing4_Na2_EDTA["Na2-EDTA"]
    CultureMech_000837 --> ing4_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing4_Na2_EDTA -.-> CHEBI_64734
    ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000837 --> ing5_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000837 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_H3BO3["H3BO3"]
    CultureMech_000837 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_000837 --> ing8_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
    CultureMech_000837 --> ing9_NiCl2_x_2_H2O
    CHEBI_34887["CHEBI:34887"]
    ing9_NiCl2_x_2_H2O -.-> CHEBI_34887
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000837 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000837 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000837 --> ing12_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing12_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000837 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_000837 --> ing14_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing14_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000837 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_Riboflavin["Riboflavin"]
    CultureMech_000837 --> ing16_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing16_Riboflavin -.-> CHEBI_17015
    ing17_Biotin["Biotin"]
    CultureMech_000837 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_000837 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_NaOH["NaOH"]
    CultureMech_000837 --> ing19_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing19_NaOH -.-> CHEBI_32145
    ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000837 --> ing20_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000837 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_KH2PO4["KH2PO4"]
    CultureMech_000837 --> ing22_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing22_KH2PO4 -.-> CHEBI_63036
    ing23_Na2HPO4["Na2HPO4"]
    CultureMech_000837 --> ing23_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing23_Na2HPO4 -.-> CHEBI_34683
    ing24_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_000837 --> ing24_FeNa_EDTA

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.1 G_PER_L CHEBI:63043
potassium nitrate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KBr
Br.K 119.002 g/mol
0.01 G_PER_L CHEBI:32030
potassium bromide
5 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
6 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.02 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 H3BO3
H3BO3 61.833 g/mol
0.02 G_PER_L CHEBI:33118
boric acid
9 Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.04 G_PER_L CHEBI:75213
sodium molybdate dihydrate
10 NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
11 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.05 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
15 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.01 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 Riboflavin
C17H20N4O6 376.369 g/mol
0.02 G_PER_L CHEBI:17015
riboflavin
18 Biotin
C10H16N2O3S 244.316 g/mol
0.001 G_PER_L CHEBI:15956
biotin
19 Vitamin B12 0.005 G_PER_L CHEBI:176843
vitamin B12
20 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
21 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 KH2PO4
H2O4P.K 136.084 g/mol
37.425 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
24 Na2HPO4
HO4P.2Na 141.958 g/mol
60.82 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 [Merged 2 duplicates: 48.95, 11.87]
25 FeNa-EDTA 4.5 G_PER_L

Preparation

  1. Prepare the medium without the vitamin solution and the phosphate buffer. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 50% methane to the gas phase and autoclave at 121°C for 15 minutes. Autoclave the phosphate buffer separately. When the growth medium is cool add 0.1 ml/l of vitamin solution (filter sterilized) and 2 ml/l of the phosphate buffer - if too warm the phosphate will precipitate. Liquid cultures should be grown with shaking.
  2. pH 6.7
  3. After the disodium phosphate has been dissolved in distilled water, pH should be between 8-9 without adjustment.
  4. Autoclave at 121 °C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.244643Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1376
2026-03-13T03:09:14.775523Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:58.776058Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000837
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.227468+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOCOCCUS MEDIUM" to "methylococcus_medium" for consistent matching
2026-06-05T07:41:33.122404+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.520663+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.555435+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.731849+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f5ffa91d02f98e8efde813c6c9075efcf6ec6fb6dca137788637b8bce7b7ea21