Overview
- External ontology
- mediadive.medium:1382 (source link unavailable) — MP MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 5.0, 'max': 6.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1382.pdf
Composition
flowchart LR
CultureMech_000842["mp_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000842 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000842 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_NH4Cl["NH4Cl"]
CultureMech_000842 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Yeast_extract["Yeast extract"]
CultureMech_000842 --> ing3_Yeast_extract
ing4_Glucose["Glucose"]
CultureMech_000842 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000842 --> ing5_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing5_FeCl2_x_4_H2O -.-> CHEBI_86249
ing6_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000842 --> ing6_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing6_CoCl2_x_6_H2O -.-> CHEBI_53503
ing7_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000842 --> ing7_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing7_NiCl2_x_6_H2O -.-> CHEBI_34887
ing8_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000842 --> ing8_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing8_CuCl2_x_2_H2O -.-> CHEBI_86318
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000842 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_ZnCl2["ZnCl2"]
CultureMech_000842 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_H3BO3["H3BO3"]
CultureMech_000842 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000842 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000842 --> ing13_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.2 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 | Yeast extract | 0.1 G_PER_L | ||
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.8 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 7 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.25 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 8 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 9 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.07 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.5 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.01 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Adjust pH to 5.0-6.5
- Hungate tubes are filled with medium by 2/3 of the total volume (e.g., 10 ml in 15 ml tubes).
- Sterilize at 121°C for 15 minutes.
- Adjust pH to 2.0 - 3.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mp_medium (CultureMech:000842)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.262318Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1382 |
| 2026-03-13T06:18:58.824420Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000842 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.280529+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MP MEDIUM" to "mp_medium" for consistent matching |
| 2026-05-20T06:02:00.059009+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '5-6.5' -> {'min': 5.0, 'max': 6.5} |
| 2026-06-05T07:41:38.288385+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.121086+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.249160+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f229dab936449fc29208f653687c06b93c505c893b013630f66f4f8b0e8f8600 |