Overview
- External ontology
- mediadive.medium:1386 (source link unavailable) — HYPHOMICROBIUM MEDIUM SAF
- Applications
-
- Microbial cultivation
- pH range
- {'min': 6.0, 'max': 6.5}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1386.pdf
Composition
flowchart LR
CultureMech_000846["hyphomicrobium_medium_saf"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000846 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_000846 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_KCl["KCl"]
CultureMech_000846 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000846 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NaCl["NaCl"]
CultureMech_000846 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000846 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_Na2SO4["Na2SO4"]
CultureMech_000846 --> ing6_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing6_Na2SO4 -.-> CHEBI_32149
ing7_Methanol["Methanol"]
CultureMech_000846 --> ing7_Methanol
CHEBI_17790["CHEBI:17790"]
ing7_Methanol -.-> CHEBI_17790
ing8_HEPES["HEPES"]
CultureMech_000846 --> ing8_HEPES
CHEBI_46756["CHEBI:46756"]
ing8_HEPES -.-> CHEBI_46756
ing9_HCl["HCl"]
CultureMech_000846 --> ing9_HCl
CHEBI_17883["CHEBI:17883"]
ing9_HCl -.-> CHEBI_17883
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000846 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_ZnCl2["ZnCl2"]
CultureMech_000846 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000846 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_H3BO3["H3BO3"]
CultureMech_000846 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000846 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000846 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000846 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000846 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_4_Aminobenzoic_Acid["4-Aminobenzoic Acid"]
CultureMech_000846 --> ing18_4_Aminobenzoic_Acid
CHEBI_30753["CHEBI:30753"]
ing18_4_Aminobenzoic_Acid -.-> CHEBI_30753
ing19_B12["B12"]
CultureMech_000846 --> ing19_B12
ing20_D_____biotin["D-(+)-biotin"]
CultureMech_000846 --> ing20_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing20_D_____biotin -.-> CHEBI_15956
ing21_Nicotinic_acid["Nicotinic acid"]
CultureMech_000846 --> ing21_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing21_Nicotinic_acid -.-> CHEBI_15940
ing22_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_000846 --> ing22_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_Calcium_D_____pantothenate -.-> CHEBI_31345
ing23_Pyridoxamine_Dihydrochloride["Pyridoxamine Dihydrochloride"]
CultureMech_000846 --> ing23_Pyridoxamine_Dihydrochloride
CHEBI_131532["CHEBI:131532"]
ing23_Pyridoxamine_Dihydrochloride -.-> CHEBI_131532
ing24_Thiamine_HCl["Thiamine HCl"]
CultureMech_000846 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.62 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.84 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 8 |
Methanol
CH4O 32.042 g/mol
|
0.000410778 G_PER_L |
CHEBI:17790
methanol
|
|
| 9 | HEPES | 4.766 G_PER_L |
CHEBI:46756
HEPES
|
|
| 10 |
HCl
HCl 36.461 g/mol
|
7.7 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
4-Aminobenzoic Acid
C7H7NO2 137.138 g/mol
|
0.04 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 20 | B12 | 0.05 G_PER_L | ||
| 21 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.1 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 23 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 24 |
Pyridoxamine Dihydrochloride
C8H12N2O2.2HCl 241.118 g/mol
|
0.1 G_PER_L |
CHEBI:131532
pyridoxamine dihydrochloride
|
|
| 25 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Preparation
- Prepare the liquid medium. Adjust to pH 6.0 – 6.5. Dispense the medium into Hungate tubes. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
- pH 6.8
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hyphomicrobium_medium_saf (CultureMech:000846)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.272832Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1386 |
| 2026-02-06T05:10:43.640281Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:18:58.857006Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000846 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.316461+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYPHOMICROBIUM MEDIUM SAF" to "hyphomicrobium_medium_saf" for consistent matching |
| 2026-05-20T06:01:23.030646+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '6-6.5' -> {'min': 6.0, 'max': 6.5} |
| 2026-06-05T07:41:09.210371+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.988656+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:38.288993+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 124e33923fd71ae34aba23d6a317a7401ee567140761d4ac0e11ad1f2a79d161 |