Overview
- External ontology
- mediadive.medium:1388 (source link unavailable) — 15% MGM-MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': '10_mgm_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1339', 'original_category': 'bacterial'}
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1388.pdf
Composition
flowchart LR
CultureMech_000848["15_mgm_medium"]
ing0_Peptone["Peptone"]
CultureMech_000848 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000848 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_000848 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_NaCl["NaCl"]
CultureMech_000848 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_000848 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000848 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000848 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaCl2["CaCl2"]
CultureMech_000848 --> ing7_CaCl2
CHEBI_3312["CHEBI:3312"]
ing7_CaCl2 -.-> CHEBI_3312
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 5 G_PER_L | ||
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
30 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
35 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.5549 G_PER_L |
CHEBI:3312
calcium dichloride
|
Preparation
- Adjust to pH 7.0 with 1 M Tris-Base and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
- Adjust to pH 7.5 with 2 M Tris-Base and sterilize by autoclaving.
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- 10_mgm_medium (CultureMech:000796)
- 15_mgm_medium (CultureMech:000848)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.278414Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1388 |
| 2026-03-13T06:18:58.869532Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000848 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.330267+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "15% MGM-MEDIUM" to "15_mgm_medium" for consistent matching |
| 2026-06-05T07:40:15.291050+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:33.128105+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: 10_mgm_medium, 15_mgm_medium |
| 2026-08-06T03:04:33.128139+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 16a0675011fbcf4230cc000aa7edf9d6d4ef63bd27ae2edd41b9515d67e3985a |