Overview
- External ontology
- mediadive.medium:1389 (source link unavailable) — LAMPROBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1389.pdf
Composition
flowchart LR
CultureMech_000849["lamprobacter_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000849 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NaCl["NaCl"]
CultureMech_000849 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_NH4Cl["NH4Cl"]
CultureMech_000849 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000849 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_NaHCO3["NaHCO3"]
CultureMech_000849 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_KCl["KCl"]
CultureMech_000849 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000849 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_000849 --> ing7_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing8_Na_acetate["Na-acetate"]
CultureMech_000849 --> ing8_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing8_Na_acetate -.-> CHEBI_32954
ing9_Yeast_extract["Yeast extract"]
CultureMech_000849 --> ing9_Yeast_extract
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_000849 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
ing11_EDTA["EDTA"]
CultureMech_000849 --> ing11_EDTA
CHEBI_4735["CHEBI:4735"]
ing11_EDTA -.-> CHEBI_4735
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000849 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000849 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000849 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_H3BO3["H3BO3"]
CultureMech_000849 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000849 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000849 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000849 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000849 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
40 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 9 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.5 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 10 | Yeast extract | 0.5 G_PER_L | ||
| 11 | Vitamin B12 | 1e-05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 12 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.03 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.5.
- Prepare the medium without the NaHCO3, Na2S x 9 H2O, Na2S2O3 x 5 H2O and vitamin B12, under a nitrogen atmosphere. Autoclave and add the vitamin B12 from a filter-sterilised stock solution and the NaHCO3, Na2S x 9 H2O, Na2S2O3 x 5 H2O from sterile stock solutions. The final pH of the medium should be 7.5.
- For Allochromatium phaeobacterium DSM 19781 reduce the amount of NaCl to 10.0 g/l and increase the amount of NH4Cl to 0.64 g/l.
- pH 3.0-4.0
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lamprobacter_medium (CultureMech:000849)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.280328Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1389 |
| 2026-03-13T06:18:58.878057Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000849 |
| 2026-03-17T06:30:15.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.339172+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LAMPROBACTER MEDIUM" to "lamprobacter_medium" for consistent matching |
| 2026-06-05T07:41:10.748301+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.476653+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.924723+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3d93012d1de7127c29d8c9ec26ecc026e8c5cfbb0a1c42ec2c3284796855958d |