mmjhsy_medium_modified

CultureMech:000852 bacterial COMPLEX LIQUID
Original name: MMJHSY MEDIUM (modified)

Overview

External ontology
mediadive.medium:1391 (source link unavailable) — MMJHSY MEDIUM (modified)
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1391.pdf

Composition

flowchart LR
    CultureMech_000852["mmjhsy_medium_modified"]
    ing0_NaCl["NaCl"]
    CultureMech_000852 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000852 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000852 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000852 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_000852 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_NaNO3["NaNO3"]
    CultureMech_000852 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000852 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000852 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_000852 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000852 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000852 --> ing10_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing11_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_000852 --> ing11_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing11_Fe_III__citrate -.-> CHEBI_144421
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_000852 --> ing12_Yeast_extract
    ing13_NaHCO3["NaHCO3"]
    CultureMech_000852 --> ing13_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing13_NaHCO3 -.-> CHEBI_32139
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000852 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_000852 --> ing15_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing15_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000852 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000852 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000852 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000852 --> ing19_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing20_H3BO3["H3BO3"]
    CultureMech_000852 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000852 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_000852 --> ing22_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing22_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing23_NaBr["NaBr"]
    CultureMech_000852 --> ing23_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing23_NaBr -.-> CHEBI_63004
    ing24_KI["KI"]
    CultureMech_000852 --> ing24_KI
    CHEBI_8346["CHEBI:8346"]
    ing24_KI -.-> CHEBI_8346
    ing25_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000852 --> ing25_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing25_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing26_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000852 --> ing26_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing26_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing27_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
    CultureMech_000852 --> ing27_Fe2_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing27_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing28_H2WO4["H2WO4"]
    CultureMech_000852 --> ing28_H2WO4
    CHEBI_36272["CHEBI:36272"]
    ing28_H2WO4 -.-> CHEBI_36272
    ing29_Biotin["Biotin"]
    CultureMech_000852 --> ing29_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing29_Biotin -.-> CHEBI_15956
    CultureMech_000852 --> more["...9 more"]

Ingredients (39)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.09 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.07 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.8 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
6 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
12 Fe(III) citrate
C6H5FeO7 244.944 g/mol
0.01 G_PER_L CHEBI:144421
iron(III) citrate
13 Yeast extract 2 G_PER_L
14 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.5 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.001 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.01 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
24 NaBr
Br.Na 102.894 g/mol
0.01 G_PER_L CHEBI:63004
sodium bromide
25 KI
I.K 166.002 g/mol
0.01 G_PER_L CHEBI:8346
potassium iodide
26 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.1 G_PER_L CHEBI:34887
nickel dichloride
27 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.1 G_PER_L CHEBI:131361
disodium selenite pentahydrate
28 Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
1 G_PER_L CHEBI:53438
iron(3+) sulfate
29 H2WO4
H2O4W 249.852 g/mol
0.1 G_PER_L CHEBI:36272
tungstic acid
30 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
31 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
32 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
33 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
34 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
35 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
36 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
37 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
38 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
39 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Prepare the medium under N2/CO2 (80%N2, 20%CO2) and fill in , the pH of the medium is adjusted with NaOH to 6.8. After autoclaving, the separately autoclaved, concentrated solutions including each of follows are added to the medium. Then a mix gas (80%H2, 20%CO2) is purged for 5 min.
  2. Finally, the mix gas (77%H2, 20%CO2, 3%O2) is compressed into gas phase (>80% volume of the tube or bottle) at 2 atm.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.287728Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1391
2026-02-06T05:10:23.380964Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:58.908511Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000852
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.371600+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJHSY MEDIUM (modified)" to "mmjhsy_medium_modified" for consistent matching
2026-06-05T07:41:35.621468+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.330621+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:47.368921+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 035f0a2bcef32f748a07d92b4c6e8e58fc601b7e78d11fd2d240b430e4d954a0