Overview
- External ontology
- mediadive.medium:1401 (source link unavailable) — MSYS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1401.pdf
Composition
flowchart LR
CultureMech_000862["msys_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000862 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NaCl["NaCl"]
CultureMech_000862 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000862 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000862 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_000862 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_Yeast_extract["Yeast extract"]
CultureMech_000862 --> ing5_Yeast_extract
ing6_Malate["Malate"]
CultureMech_000862 --> ing6_Malate
CHEBI_25115["CHEBI:25115"]
ing6_Malate -.-> CHEBI_25115
ing7_Succinate["Succinate"]
CultureMech_000862 --> ing7_Succinate
CHEBI_26806["CHEBI:26806"]
ing7_Succinate -.-> CHEBI_26806
ing8_Na2_EDTA["Na2-EDTA"]
CultureMech_000862 --> ing8_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing8_Na2_EDTA -.-> CHEBI_64734
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000862 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000862 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_MnCl2_x_2_H2O["MnCl2 x 2 H2O"]
CultureMech_000862 --> ing11_MnCl2_x_2_H2O
CHEBI_131395["CHEBI:131395"]
ing11_MnCl2_x_2_H2O -.-> CHEBI_131395
ing12_ZnCl2["ZnCl2"]
CultureMech_000862 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_000862 --> ing13_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing13_NiSO4_x_6_H2O -.-> CHEBI_53001
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000862 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_H3BO3["H3BO3"]
CultureMech_000862 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000862 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
0.2 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.045 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 6 | Yeast extract | 0.5 G_PER_L | ||
| 7 | Malate | 1 G_PER_L |
CHEBI:25115
malate
|
|
| 8 | Succinate | 1 G_PER_L |
CHEBI:26806
succinate
|
|
| 9 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.11 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
MnCl2 x 2 H2O
Cl2Mn.2H2O 161.874 g/mol
|
0.03 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.05 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.03 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Preparation
- pH 6.5
- Prepare the medium under a N2 atmosphere.
- Adjust pH to 6.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- msys_medium (CultureMech:000862)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.315850Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1401 |
| 2026-03-13T06:18:58.988099Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000862 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.454723+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MSYS MEDIUM" to "msys_medium" for consistent matching |
| 2026-06-05T07:41:38.841055+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.275499+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:50.740525+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 450ebac86b6cbfc6bbd4caa310e1fc3c718625dab89770135d9eb3e35e7e88c0 |