Overview
- External ontology
- mediadive.medium:1402 (source link unavailable) — MEIOTHERMUS MEDIUM R8
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1402.pdf
Composition
flowchart LR
CultureMech_000863["meiothermus_medium_r8"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000863 --> ing0_Yeast_extract
ing1_MgCl2_x_7_H2O["MgCl2 x 7 H2O"]
CultureMech_000863 --> ing1_MgCl2_x_7_H2O
CHEBI_6636["CHEBI:6636"]
ing1_MgCl2_x_7_H2O -.-> CHEBI_6636
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000863 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_L_Proline["L-Proline"]
CultureMech_000863 --> ing3_L_Proline
CHEBI_17203["CHEBI:17203"]
ing3_L_Proline -.-> CHEBI_17203
ing4_Agar["Agar"]
CultureMech_000863 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | ||
| 2 |
MgCl2 x 7 H2O
2Cl.Mg 95.211 g/mol
|
5.1 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
7.35075 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
L-Proline
C5H9NO2 115.132 g/mol
|
3 G_PER_L |
CHEBI:17203
L-proline
|
|
| 5 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust to pH 7.2 with NaOH (1M) and sterilize by autoclaving. The medium may be solidified by adding 20.0 g/l agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- meiothermus_medium_r8 (CultureMech:000863)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.318551Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1402 |
| 2026-01-28T19:48:26.828439Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:58.993167Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000863 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.460600+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEIOTHERMUS MEDIUM R8" to "meiothermus_medium_r8" for consistent matching |
| 2026-06-05T07:41:30.458806+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:44.446241+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 371f282b6a7a77d9ba74bf62852d8e2abd71e6590d30c86fef2b38d921356978 |