methylothermus_medium

CultureMech:000874 bacterial DEFINED LIQUID
Original name: METHYLOTHERMUS MEDIUM

Overview

External ontology
mediadive.medium:1412 (source link unavailable) — METHYLOTHERMUS MEDIUM
Applications
  • Microbial cultivation
pH
6.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1412.pdf

Composition

flowchart LR
    CultureMech_000874["methylothermus_medium"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_000874 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000874 --> ing1_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing1_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing2_NaCl["NaCl"]
    CultureMech_000874 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_000874 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000874 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000874 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000874 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_000874 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NH4Cl["NH4Cl"]
    CultureMech_000874 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_NaNO3["NaNO3"]
    CultureMech_000874 --> ing9_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing9_NaNO3 -.-> CHEBI_63005
    ing10_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_000874 --> ing10_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing10_Na2SiO3_x_9_H2O -.-> CHEBI_132108
    ing11_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
    CultureMech_000874 --> ing11_Fe2_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing11_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing12_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000874 --> ing12_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing12_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000874 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000874 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
    CultureMech_000874 --> ing15_MnSO4_x_2_H2O
    CHEBI_86356["CHEBI:86356"]
    ing15_MnSO4_x_2_H2O -.-> CHEBI_86356
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000874 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000874 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000874 --> ing18_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing19_H3BO4["H3BO4"]
    CultureMech_000874 --> ing19_H3BO4
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000874 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Biotin["Biotin"]
    CultureMech_000874 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_000874 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000874 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000874 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Riboflavin["Riboflavin"]
    CultureMech_000874 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000874 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_000874 --> ing27_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_D_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_000874 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000874 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_000874 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
2.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
2 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.259686 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
 [Merged 2 duplicates: 0.249686, 0.01]
3 NaCl
Cl.Na 58.443 g/mol
32.8805 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 3.0, 29.8805]
4 KH2PO4
H2O4P.K 136.084 g/mol
0.15344200000000002 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 [Merged 2 duplicates: 0.014, 0.139442]
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.876813 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.08, 0.796813]
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.72645 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.34, 3.38645]
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.58335 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 [Merged 2 duplicates: 0.42, 4.16335]
8 KCl
Cl.K 74.551 g/mol
33.328685 G_PER_L CHEBI:32588
potassium chloride
 [Merged 2 duplicates: 33.0, 0.328685]
9 NH4Cl
Cl.H4N 53.492 g/mol
0.499004 G_PER_L CHEBI:31206
ammonium chloride
 [Merged 2 duplicates: 0.25, 0.249004]
10 NaNO3
NO3.Na 84.994 g/mol
0.499004 G_PER_L CHEBI:63005
sodium nitrate
 [Merged 2 duplicates: 0.25, 0.249004]
11 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
0.000399203 G_PER_L CHEBI:132108
sodium silicate nonahydrate
 [Merged 2 duplicates: 0.0002, 0.000199203]
12 Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
0.0399203 G_PER_L CHEBI:53438
iron(3+) sulfate
 [Merged 2 duplicates: 0.02, 0.0199203]
13 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0009980079999999999 G_PER_L CHEBI:131361
disodium selenite pentahydrate
 [Merged 2 duplicates: 0.0005, 0.000498008]
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0009980079999999999 G_PER_L CHEBI:34887
nickel dichloride
 [Merged 2 duplicates: 0.0005, 0.000498008]
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
0.5 G_PER_L CHEBI:86356
manganese(II) sulfate dihydrate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
20 H3BO4 0.01 G_PER_L
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
23 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
27 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
28 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
29 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
30 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
31 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. or
  2. Prepare the liquid medium. Adjust to pH 6.2 with HCl or NaOH. Dispense the medium into serum bottles under gassing with N2:CO2 (80:20 v/v) for one minute. 20% methane and 5% air is added to the gas phase
  3. Adjust pH to 6.2.
  4. Dispense the medium into serum bottles under gassing with N2:CO2 (80:20 v/v) for one minute.
  5. 20% methane and 5% air is added to the gas phase.
  6. Autoclave at 121 °C for 20 min.
  7. Before inoculation add from sterile stock solutions:

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.345156Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1412
2026-01-28T19:48:22.087091Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:06.390639Z data-quality-cleanup-v1.0 Data quality fixes Merged 13 duplicate ingredient(s)
2026-03-13T06:18:59.086131Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000874
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.563628+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOTHERMUS MEDIUM" to "methylothermus_medium" for consistent matching
2026-06-05T07:41:33.443003+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.677390+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:45.150523+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3a09a3d5ff5ca452f4aa41dd2f88195ac3de80b32583e852010366a03d2aecb1