porphyrobacter_medium

CultureMech:000876 bacterial COMPLEX LIQUID
Original name: PORPHYROBACTER MEDIUM

Overview

External ontology
mediadive.medium:1414 (source link unavailable) — PORPHYROBACTER MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 8.0, 'max': 8.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1414.pdf

Composition

flowchart LR
    CultureMech_000876["porphyrobacter_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000876 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000876 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_KCl["KCl"]
    CultureMech_000876 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_Na2SO4["Na2SO4"]
    CultureMech_000876 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_Na_acetate["Na acetate"]
    CultureMech_000876 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Malic_acid["Malic acid"]
    CultureMech_000876 --> ing5_Malic_acid
    CHEBI_6650["CHEBI:6650"]
    ing5_Malic_acid -.-> CHEBI_6650
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_000876 --> ing6_Yeast_extract
    ing7_Bacto_peptone["Bacto peptone"]
    CultureMech_000876 --> ing7_Bacto_peptone
    ing8_NaHCO3["NaHCO3"]
    CultureMech_000876 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_CaCl2["CaCl2"]
    CultureMech_000876 --> ing9_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing9_CaCl2 -.-> CHEBI_3312
    ing10_KH2PO4["KH2PO4"]
    CultureMech_000876 --> ing10_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing10_KH2PO4 -.-> CHEBI_63036
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000876 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000876 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000876 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000876 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000876 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000876 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000876 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_H3BO3["H3BO3"]
    CultureMech_000876 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000876 --> ing19_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing19_Nicotinic_acid -.-> CHEBI_15940
    ing20_Thiamine["Thiamine"]
    CultureMech_000876 --> ing20_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing20_Thiamine -.-> CHEBI_18385
    ing21_Biotine["Biotine"]
    CultureMech_000876 --> ing21_Biotine
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotine -.-> CHEBI_15956
    ing22_Vitamin_B12["Vitamin B12"]
    CultureMech_000876 --> ing22_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing22_Vitamin_B12 -.-> CHEBI_176843

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.3 G_PER_L CHEBI:31206
ammonium chloride
3 KCl
Cl.K 74.551 g/mol
0.3 G_PER_L CHEBI:32588
potassium chloride
4 Na2SO4
2Na.O4S 142.043 g/mol
15 G_PER_L CHEBI:32149
sodium sulfate
5 Na acetate
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
6 Malic acid
C4H6O5 134.087 g/mol
1 G_PER_L CHEBI:6650
malic acid
7 Yeast extract 1 G_PER_L
8 Bacto peptone 0.5 G_PER_L
9 NaHCO3
CHO3.Na 84.006 g/mol
0.5 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 CaCl2
Ca.2Cl 110.984 g/mol
2 G_PER_L CHEBI:3312
calcium dichloride
11 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.3 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.003 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.005 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.001 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.002 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.005 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 H3BO3
H3BO3 61.833 g/mol
0.002 G_PER_L CHEBI:33118
boric acid
20 Nicotinic acid
C6H5NO2 123.111 g/mol
0.4 G_PER_L CHEBI:15940
nicotinic acid
21 Thiamine
C12H17N4OS 265.362 g/mol
0.4 G_PER_L CHEBI:18385
thiamine(1+)
22 Biotine
C10H16N2O3S 244.316 g/mol
0.08 G_PER_L CHEBI:15956
biotin
23 Vitamin B12 0.02 G_PER_L CHEBI:176843
vitamin B12

Preparation

  1. Adjust to pH 5.9 before autoclaving
  2. Prepare the liquid medium. Before inoculation add from sterile stock solutions:
  3. Adjust to pH 8.0 - 8.5 with 0.5 N NaOH.
  4. Adjust to pH 3.0
  5. Filtersterilized

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.353783Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1414
2026-03-13T06:18:59.106908Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000876
2026-03-17T06:29:59.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.586400+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PORPHYROBACTER MEDIUM" to "porphyrobacter_medium" for consistent matching
2026-05-20T06:02:02.813791+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '8-8.5' -> {'min': 8.0, 'max': 8.5}
2026-06-05T07:41:42.340804+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.349589+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.362720+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ec9bd47ae02e7f0453565d9aa1272b29d3e8b3b037437e50956e88ce34b5c235