Overview
- External ontology
- mediadive.medium:1417 (source link unavailable) — MMB MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1417.pdf
Composition
flowchart LR
CultureMech_000879["mmb_medium"]
ing0_Peptone["Peptone"]
CultureMech_000879 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000879 --> ing1_Yeast_extract
ing2_Ammonium_Sulfate["Ammonium Sulfate"]
CultureMech_000879 --> ing2_Ammonium_Sulfate
CHEBI_62946["CHEBI:62946"]
ing2_Ammonium_Sulfate -.-> CHEBI_62946
ing3_Glucose["Glucose"]
CultureMech_000879 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Agar["Agar"]
CultureMech_000879 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Biotin["Biotin"]
CultureMech_000879 --> ing5_Biotin
CHEBI_15956["CHEBI:15956"]
ing5_Biotin -.-> CHEBI_15956
ing6_Folic_acid["Folic acid"]
CultureMech_000879 --> ing6_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing6_Folic_acid -.-> CHEBI_27470
ing7_Thiamine_HCl["Thiamine HCl"]
CultureMech_000879 --> ing7_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing7_Thiamine_HCl -.-> CHEBI_49105
ing8_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_000879 --> ing8_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing8_D_Calcium_pantothenate -.-> CHEBI_31345
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_000879 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
ing10_Riboflavin["Riboflavin"]
CultureMech_000879 --> ing10_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing10_Riboflavin -.-> CHEBI_17015
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000879 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000879 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000879 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_Ammonium_Molybdate["Ammonium Molybdate"]
CultureMech_000879 --> ing14_Ammonium_Molybdate
CHEBI_91249["CHEBI:91249"]
ing14_Ammonium_Molybdate -.-> CHEBI_91249
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000879 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_EDTA["EDTA"]
CultureMech_000879 --> ing16_EDTA
CHEBI_4735["CHEBI:4735"]
ing16_EDTA -.-> CHEBI_4735
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000879 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_MnSO4_x_7_H2O["MnSO4 x 7 H2O"]
CultureMech_000879 --> ing18_MnSO4_x_7_H2O
CHEBI_86360["CHEBI:86360"]
ing18_MnSO4_x_7_H2O -.-> CHEBI_86360
ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000879 --> ing19_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
ing20_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_000879 --> ing20_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing20_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing21_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_000879 --> ing21_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing21_Na2B4O7_x_10_H2O -.-> CHEBI_131366
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.15 G_PER_L | ||
| 2 | Yeast extract | 0.15 G_PER_L | ||
| 3 |
Ammonium Sulfate
2H4N.O4S 132.141 g/mol
|
0.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 6 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 7 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 8 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 9 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 10 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 11 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
9.52381 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
28.2857 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.18095 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 15 |
Ammonium Molybdate
2H4N.MoO4 196.014 g/mol
|
0.00880952 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.5942857 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.0942857, 0.5] |
| 17 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.25 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
MnSO4 x 7 H2O
Mn.O4S 151.001 g/mol
|
0.154 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 20 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.04 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 21 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.025 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 22 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.018 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Preparation
- Autoclave at 121 °C for 15 min.
- Note: Dissolve and neutralize the nitrilotriacetic acid with KOH (7.3 g); add the other ingredients and adjust the pH to 6.6 - 6.8 before bringing the volume to 1.0 L with distilled water.
- Note: Initially add a few drops of H2SO4 to the distilled water to retard precipitation.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mmb_medium (CultureMech:000879)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.363461Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1417 |
| 2026-01-28T19:48:27.031337Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:15.130914Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:59.134305Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000879 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.616652+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMB MEDIUM" to "mmb_medium" for consistent matching |
| 2026-06-05T07:41:35.376438+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.252256+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.051334+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4cbdd1f06d53794e8f77f8cc9cd8dc64b5f794ce185163ac2b87bc376308fdcd |