natronorubrum_thiooxidans_medium

CultureMech:000885 archaea COMPLEX LIQUID
Original name: NATRONORUBRUM THIOOXIDANS MEDIUM

Overview

External ontology
mediadive.medium:1421 (source link unavailable) — NATRONORUBRUM THIOOXIDANS MEDIUM
Applications
  • Microbial cultivation
pH
7.5
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1421.pdf

Composition

flowchart LR
    CultureMech_000885["natronorubrum_thiooxidans_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000885 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000885 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000885 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_Na_acetate["Na-acetate"]
    CultureMech_000885 --> ing3_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Na_acetate -.-> CHEBI_32954
    ing4_MgSO4["MgSO4"]
    CultureMech_000885 --> ing4_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing4_MgSO4 -.-> CHEBI_32599
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_000885 --> ing5_Yeast_extract
    ing6_EDTA["EDTA"]
    CultureMech_000885 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000885 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_ZnSO4["ZnSO4"]
    CultureMech_000885 --> ing8_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing8_ZnSO4 -.-> CHEBI_35176
    ing9_MnCl2["MnCl2"]
    CultureMech_000885 --> ing9_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing9_MnCl2 -.-> CHEBI_63041
    ing10_H3BO3["H3BO3"]
    CultureMech_000885 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000885 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2["CuCl2"]
    CultureMech_000885 --> ing12_CuCl2
    CHEBI_49553["CHEBI:49553"]
    ing12_CuCl2 -.-> CHEBI_49553
    ing13_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
    CultureMech_000885 --> ing13_NiCl2_x_2_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_2_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000885 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
3.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
4 Na-acetate
C2H3O2.Na 82.034 g/mol
0.0262508 G_PER_L CHEBI:32954
sodium acetate
5 MgSO4
Mg.O4S 120.368 g/mol
0.00144444 G_PER_L CHEBI:32599
magnesium sulfate
6 Yeast extract 1 G_PER_L
7 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
10 MnCl2
Cl2Mn 125.844 g/mol
0.03 G_PER_L CHEBI:63041
manganese(II) chloride
11 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2
Cl2Cu 134.452 g/mol
0.01 G_PER_L CHEBI:49553
copper(II) chloride
14 NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Prepare the liquid medium. Adjust to pH 7.5. Before inoculation add from sterile stock solutions:

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.377414Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1421
2026-02-06T05:10:13.153698Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:59.224179Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000885
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.714037+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NATRONORUBRUM THIOOXIDANS MEDIUM" to "natronorubrum_thiooxidans_medium" for consistent matching
2026-06-05T07:41:39.405856+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.454529+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:32.102716+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 711c310e6908b4d787c8612ff8a52b136a7fbe7f45ef44b4bb259d8cca222350