Overview
- External ontology
- mediadive.medium:1425 (source link unavailable) — SSE/HD 1:10 MEDIUM (HEPES)
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1425.pdf
Composition
flowchart LR
CultureMech_000889["sse_hd_1_10_medium_hepes"]
ing0_Peptone["Peptone"]
CultureMech_000889 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000889 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_000889 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_HEPES["HEPES"]
CultureMech_000889 --> ing3_HEPES
CHEBI_46756["CHEBI:46756"]
ing3_HEPES -.-> CHEBI_46756
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000889 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_NH4Cl["NH4Cl"]
CultureMech_000889 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000889 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7__NH4_2SO4["(NH4)2SO4"]
CultureMech_000889 --> ing7__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing7__NH4_2SO4 -.-> CHEBI_62946
ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000889 --> ing8_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
ing9_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_000889 --> ing9_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing9_CaSO4_x_2_H2O -.-> CHEBI_32583
ing10_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_000889 --> ing10_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing10_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing11_Na_NO3["Na NO3"]
CultureMech_000889 --> ing11_Na_NO3
CHEBI_63005["CHEBI:63005"]
ing11_Na_NO3 -.-> CHEBI_63005
ing12_KH2PO4["KH2PO4"]
CultureMech_000889 --> ing12_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing12_KH2PO4 -.-> CHEBI_63036
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000889 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_K2SO4["K2SO4"]
CultureMech_000889 --> ing14_K2SO4
CHEBI_32036["CHEBI:32036"]
ing14_K2SO4 -.-> CHEBI_32036
ing15_HCl["HCl"]
CultureMech_000889 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000889 --> ing16_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
ing17_ZnCl2["ZnCl2"]
CultureMech_000889 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000889 --> ing18_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
ing19_H3BO3["H3BO3"]
CultureMech_000889 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000889 --> ing20_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000889 --> ing21_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000889 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000889 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_Biotin["Biotin"]
CultureMech_000889 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_000889 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000889 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000889 --> ing27_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing27_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing28_Riboflavin["Riboflavin"]
CultureMech_000889 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_000889 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_000889 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.5 G_PER_L | ||
| 2 | Yeast extract | 0.25 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.1 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 | HEPES | 1.95 G_PER_L |
CHEBI:46756
HEPES
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2938 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1069 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2036 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1983 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 9 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.739 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 10 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.8606 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
|
| 11 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.236 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 12 |
Na NO3
NO3.Na 84.994 g/mol
|
0.424 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 13 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.07 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0111 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
K2SO4
2K.O4S 174.259 g/mol
|
0.087 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 16 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 17 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 19 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 22 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.005 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 34 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- adjust pH to 7.0
- Add to 1000 ml of medium after autoclaving:
- Dissolve by shaking overnight. Sterilize by autoclaving.
- First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- sse_hd_1_10_medium_hepes (CultureMech:000889)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.385714Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1425 |
| 2026-01-28T19:48:20.465659Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:59.249721Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000889 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.743355+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SSE/HD 1:10 MEDIUM (HEPES)" to "sse_hd_1_10_medium_hepes" for consistent matching |
| 2026-06-05T07:41:46.878447+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.790284+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.532251+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 75f7ee4950ef52281b00c0f32ab525838238a11f2ca38a04fbeb9fd22481e1bc |