enhydrobacter_medium

CultureMech:000895 bacterial COMPLEX SOLID_AGAR
Original name: ENHYDROBACTER MEDIUM

Overview

External ontology
mediadive.medium:1432 (source link unavailable) — ENHYDROBACTER MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1432.pdf

Composition

flowchart LR
    CultureMech_000895["enhydrobacter_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_000895 --> ing0_Casamino_acids
    ing1_Glucose["Glucose"]
    CultureMech_000895 --> ing1_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing1_Glucose -.-> CHEBI_17234
    ing2_Agar["Agar"]
    CultureMech_000895 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Biotin["Biotin"]
    CultureMech_000895 --> ing3_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing3_Biotin -.-> CHEBI_15956
    ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000895 --> ing4_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000895 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000895 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000895 --> ing7_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000895 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Na_EDTA["Na-EDTA"]
    CultureMech_000895 --> ing9_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na_EDTA -.-> CHEBI_64734
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000895 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000895 --> ing11_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing11_MnSO4_x_H2O -.-> CHEBI_86364
    ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000895 --> ing12_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing13_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000895 --> ing13_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing13_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing14_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_000895 --> ing14_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing14_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Casamino acids 1 G_PER_L
2 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
3 Agar 15 G_PER_L CHEBI:2509
agar
4 Biotin
C10H16N2O3S 244.316 g/mol
2e-05 G_PER_L CHEBI:15956
biotin
5 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.34 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01267 G_PER_L CHEBI:75213
sodium molybdate dihydrate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
10 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
13 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
14 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
15 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. Sterilize 20 min. /121°C. After cooling to 60°C add to the medium:
  2. Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.
  3. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.403062Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1432
2026-01-28T19:48:38.747152Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:36.843697Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:59.305174Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000895
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.806779+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ENHYDROBACTER MEDIUM" to "enhydrobacter_medium" for consistent matching
2026-06-05T07:40:59.569977+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:38.124598+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-08-06T03:05:33.599316+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b9daac43c4f69c76efc2b935440b82d0e7ceb9945e021031db7e1c2e581b52b2