Overview
- External ontology
- mediadive.medium:1435 (source link unavailable) — METHYLOTROPHES MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1435.pdf
Composition
flowchart LR
CultureMech_000897["methylotrophes_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_000897 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000897 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_NH4Cl["NH4Cl"]
CultureMech_000897 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000897 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Yeast_extract["Yeast extract"]
CultureMech_000897 --> ing4_Yeast_extract
ing5_Disodium_succinate["Disodium succinate"]
CultureMech_000897 --> ing5_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing5_Disodium_succinate -.-> CHEBI_63675
ing6_Fe_III__citrate["Fe(III) citrate"]
CultureMech_000897 --> ing6_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing6_Fe_III__citrate -.-> CHEBI_144421
ing7_Ethanol["Ethanol"]
CultureMech_000897 --> ing7_Ethanol
CHEBI_16236["CHEBI:16236"]
ing7_Ethanol -.-> CHEBI_16236
ing8_Agar["Agar"]
CultureMech_000897 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 | Yeast extract | 2 G_PER_L | ||
| 6 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
2 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 7 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
5 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 8 |
Ethanol
C2H6O 46.069 g/mol
|
0.5 G_PER_L |
CHEBI:16236
ethanol
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Prepare the medium without ethanol in closed vessels with a medium to headspace ratio of 1:5 to 1:10. Adjust pH to 7.0 and sterilize. Allow to cool to about 45°C before adding 0.5 mL filter-sterilized ethanol per liter medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methylotrophes_medium (CultureMech:000897)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.410750Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1435 |
| 2026-03-13T06:18:59.319118Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000897 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:10.822892+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOTROPHES MEDIUM" to "methylotrophes_medium" for consistent matching |
| 2026-06-05T07:41:33.486729+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.698166+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:45.204653+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint da7235f20aa3217c400b470c5f861ee804c05944c437954c492accb8c4f2f2ce |