alkaliphiles_pirellula_medium_m1

CultureMech:000904 bacterial COMPLEX LIQUID
Original name: ALKALIPHILES PIRELLULA MEDIUM (M1)

Overview

External ontology
mediadive.medium:1441 (source link unavailable) — ALKALIPHILES PIRELLULA MEDIUM (M1)
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1441.pdf

Composition

flowchart LR
    CultureMech_000904["alkaliphiles_pirellula_medium_m1"]
    ing0_CaCO3["CaCO3"]
    CultureMech_000904 --> ing0_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing0_CaCO3 -.-> CHEBI_3311
    ing1_Na2HPO4_x_H2O["Na2HPO4 x H2O"]
    CultureMech_000904 --> ing1_Na2HPO4_x_H2O
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4_x_H2O -.-> CHEBI_34683
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000904 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Gelrite["Gelrite"]
    CultureMech_000904 --> ing3_Gelrite
    CHEBI_85248["CHEBI:85248"]
    ing3_Gelrite -.-> CHEBI_85248
    ing4_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_000904 --> ing4_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing4_N_Acetylglucosamine -.-> CHEBI_59640
    ing5_Vitamin_B12["Vitamin B12"]
    CultureMech_000904 --> ing5_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing5_Vitamin_B12 -.-> CHEBI_176843
    ing6_Biotin["Biotin"]
    CultureMech_000904 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000904 --> ing7_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing8_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000904 --> ing8_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing8_Calcium_pantothenate -.-> CHEBI_31345
    ing9_Folic_acid["Folic acid"]
    CultureMech_000904 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Riboflavin["Riboflavin"]
    CultureMech_000904 --> ing10_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing10_Riboflavin -.-> CHEBI_17015
    ing11_Nicotinamide["Nicotinamide"]
    CultureMech_000904 --> ing11_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing11_Nicotinamide -.-> CHEBI_17154
    ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000904 --> ing12_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000904 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000904 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000904 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000904 --> ing16__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing16__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000904 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_Na_EDTA["Na-EDTA"]
    CultureMech_000904 --> ing18_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing18_Na_EDTA -.-> CHEBI_64734
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000904 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_000904 --> ing20_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing20_MnSO4_x_H2O -.-> CHEBI_86364
    ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000904 --> ing21_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing22_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000904 --> ing22_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing22_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing23_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_000904 --> ing23_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing23_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 CaCO3
CO3.Ca 100.086 g/mol
5.20833 G_PER_L CHEBI:3311
calcium carbonate
2 Na2HPO4 x H2O
HO4P.2Na 141.958 g/mol
0.104167 G_PER_L CHEBI:34683
disodium hydrogenphosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
30.220833 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.520833, 29.7]
4 Gelrite 9.375 G_PER_L CHEBI:85248
gellan gum
5 N-Acetylglucosamine 40 G_PER_L CHEBI:59640
N-acetylglucosamine
6 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
7 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
8 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
9 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
10 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
11 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
12 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
13 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
17 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
19 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
21 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
22 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
23 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
24 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. Adjust pH to 9.0, autoclave at 121°C for 20 min.
  2. Adjust pH to 9.0, filter-sterilize, and add to solution 1.
  3. filter-sterilized
  4. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
  5. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.435856Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1441
2026-03-13T03:08:55.747825Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:59.408470Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000904
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.916685+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALIPHILES PIRELLULA MEDIUM (M1)" to "alkaliphiles_pirellula_medium_m1" for consistent matching
2026-06-05T07:40:47.965493+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.821600+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:22.466067+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0f6de048eccbc9bb971d95acbf94cb96d2235b80710bf66256680ef72ba2a7b2