pirellula_medium_m33a

CultureMech:000905 bacterial COMPLEX SOLID_AGAR
Original name: PIRELLULA MEDIUM (M33a)

Overview

External ontology
mediadive.medium:1442 (source link unavailable) — PIRELLULA MEDIUM (M33a)
Applications
  • Microbial cultivation
pH range
{'min': 7.5, 'max': 8.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1442.pdf

Composition

flowchart LR
    CultureMech_000905["pirellula_medium_m33a"]
    ing0_Gelatine["Gelatine"]
    CultureMech_000905 --> ing0_Gelatine
    CHEBI_5291["CHEBI:5291"]
    ing0_Gelatine -.-> CHEBI_5291
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000905 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_000905 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_000905 --> ing3_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing3_Tris_HCl_buffer -.-> CHEBI_9754
    ing4_Agar["Agar"]
    CultureMech_000905 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509
    ing5_Vitamin_B12["Vitamin B12"]
    CultureMech_000905 --> ing5_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing5_Vitamin_B12 -.-> CHEBI_176843
    ing6_Biotin["Biotin"]
    CultureMech_000905 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000905 --> ing7_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing7_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing8_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000905 --> ing8_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing8_Calcium_pantothenate -.-> CHEBI_31345
    ing9_Folic_acid["Folic acid"]
    CultureMech_000905 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Riboflavin["Riboflavin"]
    CultureMech_000905 --> ing10_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing10_Riboflavin -.-> CHEBI_17015
    ing11_Nicotinamide["Nicotinamide"]
    CultureMech_000905 --> ing11_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing11_Nicotinamide -.-> CHEBI_17154
    ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000905 --> ing12_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000905 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_NaCl["NaCl"]
    CultureMech_000905 --> ing14_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing14_NaCl -.-> CHEBI_26710
    ing15_Na2SO4["Na2SO4"]
    CultureMech_000905 --> ing15_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing15_Na2SO4 -.-> CHEBI_32149
    ing16_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000905 --> ing16_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing16_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing17_CaCl2["CaCl2"]
    CultureMech_000905 --> ing17_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing17_CaCl2 -.-> CHEBI_3312
    ing18_NaHCO3["NaHCO3"]
    CultureMech_000905 --> ing18_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing18_NaHCO3 -.-> CHEBI_32139
    ing19_KCl["KCl"]
    CultureMech_000905 --> ing19_KCl
    CHEBI_32588["CHEBI:32588"]
    ing19_KCl -.-> CHEBI_32588
    ing20_KBr["KBr"]
    CultureMech_000905 --> ing20_KBr
    CHEBI_32030["CHEBI:32030"]
    ing20_KBr -.-> CHEBI_32030
    ing21_H3BO3["H3BO3"]
    CultureMech_000905 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_SrCl2["SrCl2"]
    CultureMech_000905 --> ing22_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing22_SrCl2 -.-> CHEBI_36383
    ing23_NaF["NaF"]
    CultureMech_000905 --> ing23_NaF
    CHEBI_28741["CHEBI:28741"]
    ing23_NaF -.-> CHEBI_28741
    ing24_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000905 --> ing24_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing24_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing25_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000905 --> ing25_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing25_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing26_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000905 --> ing26_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing26_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing27__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000905 --> ing27__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing27__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing28_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000905 --> ing28_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing28_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing29_Na_EDTA["Na-EDTA"]
    CultureMech_000905 --> ing29_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing29_Na_EDTA -.-> CHEBI_64734
    CultureMech_000905 --> more["...5 more"]

Ingredients (35)

# Ingredient Concentration Ontology Notes
1 Gelatine 2 G_PER_L CHEBI:5291
gelatin
2 Yeast extract 0.25 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
4 Tris-HCl buffer
C4H11NO3 121.136 g/mol
50 G_PER_L CHEBI:9754
tris
5 Agar 18 G_PER_L CHEBI:2509
agar
6 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
7 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
8 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
9 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
10 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
11 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
12 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
13 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
14 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
15 NaCl
Cl.Na 58.443 g/mol
23.47 G_PER_L CHEBI:26710
sodium chloride
16 Na2SO4
2Na.O4S 142.043 g/mol
3.92 G_PER_L CHEBI:32149
sodium sulfate
17 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.98 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
18 CaCl2
Ca.2Cl 110.984 g/mol
1.1 G_PER_L CHEBI:3312
calcium dichloride
19 NaHCO3
CHO3.Na 84.006 g/mol
0.192 G_PER_L CHEBI:32139
sodium hydrogencarbonate
20 KCl
Cl.K 74.551 g/mol
0.664 G_PER_L CHEBI:32588
potassium chloride
21 KBr
Br.K 119.002 g/mol
0.096 G_PER_L CHEBI:32030
potassium bromide
22 H3BO3
H3BO3 61.833 g/mol
0.026 G_PER_L CHEBI:33118
boric acid
23 SrCl2
2Cl.Sr 158.526 g/mol
0.024 G_PER_L CHEBI:36383
strontium dichloride
24 NaF
F.Na 41.988 g/mol
0.003 G_PER_L CHEBI:28741
sodium fluoride
25 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
26 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
27 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
28 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
29 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
30 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
31 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
32 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
33 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
34 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
35 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. Adjust pH to 7.5, autoclave at 121°C for 20 min.
  2. For agar plates increase the pH of the Tris/HCl buffer to 8.5.
  3. filter-sterilized
  4. Dissolve the nitrilotriacetic acid.
  5. Adjust pH to 7.0 with KOH (~7.3 g).
  6. Dissolve other salts separately and combine.
  7. Adjust pH to 6.8 with NaOH or H2SO4.
  8. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.439865Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1442
2026-03-13T03:08:55.852502Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:59.423386Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000905
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:10.932583+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PIRELLULA MEDIUM (M33a)" to "pirellula_medium_m33a" for consistent matching
2026-05-20T06:02:02.591388+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.5-8.5' -> {'min': 7.5, 'max': 8.5}
2026-06-05T07:41:42.026719+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.260914+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.050480+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a423c7fa3be342639e1840b5450e6d59716060953061f30dd8390664eb41b2d6