meiothermus_hypogaeus_medium

CultureMech:000920 bacterial COMPLEX SOLID_AGAR
Original name: Meiothermus hypogaeus MEDIUM

Overview

External ontology
mediadive.medium:1454 (source link unavailable) — Meiothermus hypogaeus MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1454.pdf

Composition

flowchart LR
    CultureMech_000920["meiothermus_hypogaeus_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000920 --> ing0_Yeast_extract
    ing1_Bacto_Tryptone["Bacto Tryptone"]
    CultureMech_000920 --> ing1_Bacto_Tryptone
    ing2_Sodium_glutamate_monohydrate["Sodium glutamate monohydrate"]
    CultureMech_000920 --> ing2_Sodium_glutamate_monohydrate
    CHEBI_232425["CHEBI:232425"]
    ing2_Sodium_glutamate_monohydrate -.-> CHEBI_232425
    ing3_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_000920 --> ing3_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing3_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000920 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_000920 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_KNO3["KNO3"]
    CultureMech_000920 --> ing6_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing6_KNO3 -.-> CHEBI_63043
    ing7_NaNO3["NaNO3"]
    CultureMech_000920 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_Na2HPO4["Na2HPO4"]
    CultureMech_000920 --> ing8_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing8_Na2HPO4 -.-> CHEBI_34683
    ing9_Agar["Agar"]
    CultureMech_000920 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_000920 --> ing10_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing11_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000920 --> ing11_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing11_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000920 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000920 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000920 --> ing14_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing15_ZnCl2["ZnCl2"]
    CultureMech_000920 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000920 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_H3BO3["H3BO3"]
    CultureMech_000920 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000920 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000920 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_Na2SeO4["Na2SeO4"]
    CultureMech_000920 --> ing20_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing20_Na2SeO4 -.-> CHEBI_77775
    ing21_Na2WO4["Na2WO4"]
    CultureMech_000920 --> ing21_Na2WO4
    CHEBI_63940["CHEBI:63940"]
    ing21_Na2WO4 -.-> CHEBI_63940

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 Yeast extract 1 G_PER_L
2 Bacto Tryptone 1 G_PER_L
3 Sodium glutamate monohydrate
C5H8NO4.H2O.Na 187.127 g/mol
1 G_PER_L CHEBI:232425
monosodium L-glutamate hydrate
4 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.06 G_PER_L CHEBI:32583
calcium sulfate dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 NaCl
Cl.Na 58.443 g/mol
1.008 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.008, 1.0]
7 KNO3
K.NO3 101.102 g/mol
0.1 G_PER_L CHEBI:63043
potassium nitrate
8 NaNO3
NO3.Na 84.994 g/mol
0.69 G_PER_L CHEBI:63005
sodium nitrate
9 Na2HPO4
HO4P.2Na 141.958 g/mol
0.11 G_PER_L CHEBI:34683
disodium hydrogenphosphate
10 Agar 20 G_PER_L CHEBI:2509
agar
11 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
12 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride
21 Na2SeO4
2Na.O4Se 188.936 g/mol
0.004 G_PER_L CHEBI:77775
sodium selenate
22 Na2WO4
2Na.O4W 293.816 g/mol
0.004 G_PER_L CHEBI:63940
sodium tungstate

Preparation

  1. Adjust pH to 7.5 with NaOH.
  2. First dissolve the nitrilotriacetic acid and adjust pH to 6.5 with NaOH, and then add minerals. Final pH 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.478697Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1454
2026-03-13T03:09:03.271445Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:59.572043Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000920
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.096284+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Meiothermus hypogaeus MEDIUM" to "meiothermus_hypogaeus_medium" for consistent matching
2026-06-05T07:41:30.451419+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 17 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.449406+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:06.566597+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.433030+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3e36c3ac46db89d987693055daabc0058989b3bacd9521d851e4ce58aa2d6ddc