Overview
- External ontology
- mediadive.medium:1461 (source link unavailable) — SALINIVIBRIO SHARMENSIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1461.pdf
Composition
flowchart LR
CultureMech_000927["salinivibrio_sharmensis_medium"]
ing0_NaCl["NaCl"]
CultureMech_000927 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_Trisodium_citrate["Trisodium citrate"]
CultureMech_000927 --> ing1_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate -.-> CHEBI_53258
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000927 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_000927 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Na2CO3["Na2CO3"]
CultureMech_000927 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000927 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000927 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Yeast_extract["Yeast extract"]
CultureMech_000927 --> ing7_Yeast_extract
ing8_Agar["Agar"]
CultureMech_000927 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
100 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
3 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 | Yeast extract | 10 G_PER_L | ||
| 9 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Final pH 9.0
- Add all the components together, except Na2CO3 which is added from a sterilized stock solution when the autoclaved medium has cooled. Agar is added at a concentration of 20 g/l for solid media and should be cooled to about 55°C before the Na2CO3.is added.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- salinivibrio_sharmensis_medium (CultureMech:000927)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.504003Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1461 |
| 2026-03-13T06:18:59.640651Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000927 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.171608+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SALINIVIBRIO SHARMENSIS MEDIUM" to "salinivibrio_sharmensis_medium" for consistent matching |
| 2026-06-05T07:41:45.130213+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.263752+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.201557+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c81ce21bd226f9a3ec4831d6be9c768c8a5164a3fc1f662b07da9a0dffe6a7a1 |