Overview
- External ontology
- mediadive.medium:1468 (source link unavailable) — HALORHABDUS SEGORENSIS S21 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1468.pdf
Composition
flowchart LR
CultureMech_000934["halorhabdus_segorensis_s21_medium"]
ing0_NaCl["NaCl"]
CultureMech_000934 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000934 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000934 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_000934 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Yeast_extract["Yeast extract"]
CultureMech_000934 --> ing4_Yeast_extract
ing5_Casamino_acids["Casamino acids"]
CultureMech_000934 --> ing5_Casamino_acids
ing6_Starch["Starch"]
CultureMech_000934 --> ing6_Starch
CHEBI_28017["CHEBI:28017"]
ing6_Starch -.-> CHEBI_28017
ing7_Agar["Agar"]
CultureMech_000934 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
41 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
203 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
29.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
7.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 | Yeast extract | 1 G_PER_L | ||
| 6 | Casamino acids | 1 G_PER_L | ||
| 7 | Starch | 2 G_PER_L |
CHEBI:28017
starch
|
|
| 8 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Before autoclaving adjust pH to 7.0 with NaOH.
- For solid medium sterilize the MgCl2 x 6 H2O dissolved in 500 ml distilled water by autoclaving. Dissolve the remaining ingredients in 500 ml distilled water (including 20.0 g agar), sterilise by autoclaving, cool to about 50°C and then mix with the warm sterile MgCl2 solution before pouring the plates.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- halorhabdus_segorensis_s21_medium (CultureMech:000934)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.519500Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1468 |
| 2026-01-28T19:48:12.696486Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:59.683460Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000934 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.219706+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALORHABDUS SEGORENSIS S21 MEDIUM" to "halorhabdus_segorensis_s21_medium" for consistent matching |
| 2026-06-05T07:41:08.114609+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.780917+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:04:28.911381+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 88c289ec8378d059816bd02ab8caab73d52cf979d424727c63b700762a42fc3f |