halodesulfuriarchaeum_medium

CultureMech:000946 bacterial COMPLEX LIQUID
Original name: HALODESULFURIARCHAEUM MEDIUM

Overview

External ontology
mediadive.medium:1479 (source link unavailable) — HALODESULFURIARCHAEUM MEDIUM
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1479.pdf

Composition

flowchart LR
    CultureMech_000946["halodesulfuriarchaeum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000946 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000946 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000946 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_HEPES["HEPES"]
    CultureMech_000946 --> ing3_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing3_HEPES -.-> CHEBI_46756
    ing4_Sulfur_powder["Sulfur powder"]
    CultureMech_000946 --> ing4_Sulfur_powder
    CHEBI_33403["CHEBI:33403"]
    ing4_Sulfur_powder -.-> CHEBI_33403
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000946 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_NaHCO3["NaHCO3"]
    CultureMech_000946 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Sodium_formate["Sodium formate"]
    CultureMech_000946 --> ing7_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing7_Sodium_formate -.-> CHEBI_62965
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_000946 --> ing8_Yeast_extract
    ing9_Disodium_sulfide["Disodium sulfide"]
    CultureMech_000946 --> ing9_Disodium_sulfide
    CHEBI_76208["CHEBI:76208"]
    ing9_Disodium_sulfide -.-> CHEBI_76208
    ing10_EDTA["EDTA"]
    CultureMech_000946 --> ing10_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing10_EDTA -.-> CHEBI_4735
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000946 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_ZnSO4["ZnSO4"]
    CultureMech_000946 --> ing12_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing12_ZnSO4 -.-> CHEBI_35176
    ing13_MnCl2["MnCl2"]
    CultureMech_000946 --> ing13_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing13_MnCl2 -.-> CHEBI_63041
    ing14_H3BO3["H3BO3"]
    CultureMech_000946 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000946 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_000946 --> ing16_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing16_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000946 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000946 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Thiamin["Thiamin"]
    CultureMech_000946 --> ing19_Thiamin
    CHEBI_18385["CHEBI:18385"]
    ing19_Thiamin -.-> CHEBI_18385
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000946 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_Biotin["Biotin"]
    CultureMech_000946 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_PABA["PABA"]
    CultureMech_000946 --> ing22_PABA
    CHEBI_30753["CHEBI:30753"]
    ing22_PABA -.-> CHEBI_30753
    ing23_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000946 --> ing23_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing23_Nicotinic_acid -.-> CHEBI_15940
    ing24_Pyridoxine["Pyridoxine"]
    CultureMech_000946 --> ing24_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing24_Pyridoxine -.-> CHEBI_16709
    ing25_Folic_acid["Folic acid"]
    CultureMech_000946 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Riboflavin["Riboflavin"]
    CultureMech_000946 --> ing26_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing26_Riboflavin -.-> CHEBI_17015
    ing27_B12["B12"]
    CultureMech_000946 --> ing27_B12

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
240 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
4 HEPES 10 G_PER_L CHEBI:46756
HEPES
5 Sulfur powder 2 G_PER_L CHEBI:33403
elemental sulfur
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.609909 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 NaHCO3
CHO3.Na 84.006 g/mol
0.420035 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 Sodium formate
CHO2.Na 68.007 g/mol
3.40035 G_PER_L CHEBI:62965
sodium formate
9 Yeast extract 0.2 G_PER_L
10 Disodium sulfide
2Na.S 78.047 g/mol
1 MILLIMOLAR CHEBI:76208
sodium sulfide (anhydrous)
11 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
14 MnCl2
Cl2Mn 125.844 g/mol
0.03 G_PER_L CHEBI:63041
manganese(II) chloride
15 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.01 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Thiamin
C12H17N4OS 265.362 g/mol
0.0125 G_PER_L CHEBI:18385
thiamine(1+)
21 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0125 G_PER_L CHEBI:31345
Calcium pantothenate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.005 G_PER_L CHEBI:15956
biotin
23 PABA
C7H7NO2 137.138 g/mol
0.0125 G_PER_L CHEBI:30753
4-aminobenzoic acid
24 Nicotinic acid
C6H5NO2 123.111 g/mol
0.0125 G_PER_L CHEBI:15940
nicotinic acid
25 Pyridoxine
C8H11NO3 169.18 g/mol
0.025 G_PER_L CHEBI:16709
pyridoxine
26 Folic acid
C19H19N7O6 441.404 g/mol
0.005 G_PER_L CHEBI:27470
folic acid
27 Riboflavin
C17H20N4O6 376.369 g/mol
0.0125 G_PER_L CHEBI:17015
riboflavin
28 B12 0.00125 G_PER_L

Preparation

  1. Prepare the liquid medium. Dispense the medium into Hungate tubes or serum bottles under gassing with N2. Steam medium for 3h on each of 3 successive days. Before inoculation add from sterile stock solutions:
  2. Adjust pH to 3.0-4.0 with HCl.
  3. Autoclave at 121 °C for 15 min.
  4. Note: autoclave in closed bottles
  5. 1: acidic solution (HCl, pH3; 100ml)
  6. 2: basic solution (100ml 0.1N NaOH)
  7. 3: Neutral solution in 100 ml demi water
  8. 4: neutral solution in 100 ml demi water
  9. Sterilize by filtration.
  10. Mix 1:1:1:1 just after addition or add separatlely, each 1ml/L.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:14.556838Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1479
2026-02-06T05:10:14.197831Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:59.807360Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000946
2026-03-17T06:29:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.356185+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALODESULFURIARCHAEUM MEDIUM" to "halodesulfuriarchaeum_medium" for consistent matching
2026-06-05T07:41:07.766139+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.512504+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:37.158378+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 13fcab0a2d470e773315eb9776d12adf300d4033470e0c52585c74cbef113621