Overview
- External ontology
- mediadive.medium:1480 (source link unavailable) — MODIFIED TRYPTONE SOYA BROTH
- Applications
-
- Microbial cultivation
- pH
- 10.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1480.pdf Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_000948["modified_tryptone_soya_broth"]
ing0_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_000948 --> ing0_Pancreatic_digest_of_casein
ing1_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_000948 --> ing1_Peptic_digest_of_soybean_meal
ing2_Glucose["Glucose"]
CultureMech_000948 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_000948 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_000948 --> ing4_Dipotassium_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Dipotassium_phosphate -.-> CHEBI_131527
ing5_Agar["Agar"]
CultureMech_000948 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_NaCl["NaCl"]
CultureMech_000948 --> ing6_NaCl
ing6_NaCl -.-> CHEBI_26710
ing7_NaCO3["NaCO3"]
CultureMech_000948 --> ing7_NaCO3
CHEBI_29377["CHEBI:29377"]
ing7_NaCO3 -.-> CHEBI_29377
ing8_Agar["Agar"]
CultureMech_000948 --> ing8_Agar
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 2 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 3 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 5 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 6 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
40 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
NaCO3
CO3.2Na 105.988 g/mol
|
2.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- The pH should be 10.5 after autoclaving
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- DSMZ_1480_MODIFIED_TRYPTONE_SOYA_BROTH (CultureMech:000948)
- modified_tryptone_soya_broth (CultureMech:000939)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.561087Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1480 |
| 2026-01-28T19:48:15.950026Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:59.820142Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000948 |
| 2026-03-17T06:29:59.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.371267+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED TRYPTONE SOYA BROTH" to "modified_tryptone_soya_broth" for consistent matching |
| 2026-06-05T07:40:16.067329+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:34.509874+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: DSMZ_1480_MODIFIED_TRYPTONE_SOYA_BROTH, modified_tryptone_soya_broth |
| 2026-08-06T03:04:34.509914+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 5f8fed0a49953df555afb2d222e5f521f7b8b98315ff542349183d3d83f98530 |