Overview
- External ontology
- mediadive.medium:1481 (source link unavailable) — HALOFERAX MEDIUM RS75
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1481.pdf
Composition
flowchart LR
CultureMech_000949["haloferax_medium_rs75"]
ing0_NaCl["NaCl"]
CultureMech_000949 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000949 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_K2SO4["K2SO4"]
CultureMech_000949 --> ing2_K2SO4
CHEBI_32036["CHEBI:32036"]
ing2_K2SO4 -.-> CHEBI_32036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000949 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Na_pyruvate["Na pyruvate"]
CultureMech_000949 --> ing4_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Na_pyruvate -.-> CHEBI_50144
ing5_Na_acetate_x_3_H2O["Na acetate x 3 H2O"]
CultureMech_000949 --> ing5_Na_acetate_x_3_H2O
ing6_Na_malate["Na malate"]
CultureMech_000949 --> ing6_Na_malate
CHEBI_91260["CHEBI:91260"]
ing6_Na_malate -.-> CHEBI_91260
ing7_Yeast_extract["Yeast extract"]
CultureMech_000949 --> ing7_Yeast_extract
ing8_Tryptone["Tryptone"]
CultureMech_000949 --> ing8_Tryptone
ing9_Glucose["Glucose"]
CultureMech_000949 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_Agar["Agar"]
CultureMech_000949 --> ing10_Agar
CHEBI_2509["CHEBI:2509"]
ing10_Agar -.-> CHEBI_2509
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
160 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
50 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
Na pyruvate
C3H3O3.Na 110.044 g/mol
|
2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 6 | Na acetate x 3 H2O | 0.5 G_PER_L | ||
| 7 | Na malate | 0.5 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 8 | Yeast extract | 1 G_PER_L | ||
| 9 | Tryptone | 0.5 G_PER_L | ||
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 11 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust to pH 6.8
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- haloferax_medium_rs75 (CultureMech:000949)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.562441Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1481 |
| 2026-03-13T06:18:59.824860Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000949 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.376649+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOFERAX MEDIUM RS75" to "haloferax_medium_rs75" for consistent matching |
| 2026-06-05T07:41:07.782776+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.518758+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.771490+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 103838b58b1b7f510df17d0a293384e95a3fc4394f33be4905081cff92046ff9 |