Overview
- External ontology
- mediadive.medium:1485 (source link unavailable) — DESULFONATRONUM ALKALITOLERANS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 8.7, 'max': 9.0}
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1485.pdf
Composition
flowchart LR
CultureMech_000952["desulfonatronum_alkalitolerans_medium"]
ing0_NaCl["NaCl"]
CultureMech_000952 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_000952 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NaHCO3["NaHCO3"]
CultureMech_000952 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_Na2CO3["Na2CO3"]
CultureMech_000952 --> ing3_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing3_Na2CO3 -.-> CHEBI_29377
ing4_Na2SO4["Na2SO4"]
CultureMech_000952 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_Na_acetate["Na-acetate"]
CultureMech_000952 --> ing5_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Na_acetate -.-> CHEBI_32954
ing6_NH4Cl["NH4Cl"]
CultureMech_000952 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000952 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_Yeast_extract["Yeast extract"]
CultureMech_000952 --> ing8_Yeast_extract
ing9_Na_formate["Na-formate"]
CultureMech_000952 --> ing9_Na_formate
CHEBI_62965["CHEBI:62965"]
ing9_Na_formate -.-> CHEBI_62965
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_000952 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_EDTA["EDTA"]
CultureMech_000952 --> ing11_EDTA
CHEBI_4735["CHEBI:4735"]
ing11_EDTA -.-> CHEBI_4735
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000952 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000952 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000952 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_H3BO3["H3BO3"]
CultureMech_000952 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000952 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_000952 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000952 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000952 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_NaOH["NaOH"]
CultureMech_000952 --> ing20_NaOH
CHEBI_32145["CHEBI:32145"]
ing20_NaOH -.-> CHEBI_32145
ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000952 --> ing21_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000952 --> ing22_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
6.31579 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.05263 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
33.6842 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
Na2CO3
CO3.2Na 105.988 g/mol
|
11.1579 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.94737 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.210526 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
20 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 | Yeast extract | 2 G_PER_L | ||
| 10 |
Na-formate
CHO2.Na 68.007 g/mol
|
340 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.03 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 22 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 23 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients of solution A except carbonates, sparge with 100% N2 gas for 30 - 45 min to make it anoxic, then add bicarbonate and carbonate, adjust pH to 9.0, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions B to H prepared under 100% N2 gas atmosphere. Adjust pH of the complete medium to 8.7 - 9.0, if necessary.
- pH 3.0-4.0
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfonatronum_alkalitolerans_medium (CultureMech:000952)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.574227Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1485 |
| 2026-03-13T06:18:59.849622Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000952 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.404613+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFONATRONUM ALKALITOLERANS MEDIUM" to "desulfonatronum_alkalitolerans_medium" for consistent matching |
| 2026-05-20T06:01:14.076756+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '8.7-9' -> {'min': 8.7, 'max': 9.0} |
| 2026-06-05T07:40:56.888748+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.686613+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 7 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:31.516480+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7bcf767701fb6d6def417bcdedf5f29eed41a1c6be55451984c086788932b100 |