cyclonatronum_proteivorans_medium_omega

CultureMech:000959 bacterial COMPLEX LIQUID
Original name: CYCLONATRONUM PROTEIVORANS MEDIUM (OMEGA)

Overview

External ontology
mediadive.medium:1491 (source link unavailable) — CYCLONATRONUM PROTEIVORANS MEDIUM (OMEGA)
Applications
  • Microbial cultivation
pH range
{'min': 10.0, 'max': 10.1}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1491.pdf

Composition

flowchart LR
    CultureMech_000959["cyclonatronum_proteivorans_medium_omega"]
    ing0_Na2CO3["Na2CO3"]
    CultureMech_000959 --> ing0_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing0_Na2CO3 -.-> CHEBI_29377
    ing1_NaCl["NaCl"]
    CultureMech_000959 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000959 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_000959 --> ing3_Yeast_extract
    ing4_Peptone["Peptone"]
    CultureMech_000959 --> ing4_Peptone
    ing5_EDTA["EDTA"]
    CultureMech_000959 --> ing5_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing5_EDTA -.-> CHEBI_4735
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000959 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_ZnSO4["ZnSO4"]
    CultureMech_000959 --> ing7_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing7_ZnSO4 -.-> CHEBI_35176
    ing8_MnCl2["MnCl2"]
    CultureMech_000959 --> ing8_MnCl2
    CHEBI_63041["CHEBI:63041"]
    ing8_MnCl2 -.-> CHEBI_63041
    ing9_H3BO3["H3BO3"]
    CultureMech_000959 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000959 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_CuCl2_x_5_H2O["CuCl2 x 5 H2O"]
    CultureMech_000959 --> ing11_CuCl2_x_5_H2O
    CHEBI_91245["CHEBI:91245"]
    ing11_CuCl2_x_5_H2O -.-> CHEBI_91245
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000959 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000959 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Thiamin["Thiamin"]
    CultureMech_000959 --> ing14_Thiamin
    CHEBI_18385["CHEBI:18385"]
    ing14_Thiamin -.-> CHEBI_18385
    ing15_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000959 --> ing15_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_pantothenate -.-> CHEBI_31345
    ing16_Biotin["Biotin"]
    CultureMech_000959 --> ing16_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotin -.-> CHEBI_15956
    ing17_PABA["PABA"]
    CultureMech_000959 --> ing17_PABA
    CHEBI_30753["CHEBI:30753"]
    ing17_PABA -.-> CHEBI_30753
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000959 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Pyridoxine["Pyridoxine"]
    CultureMech_000959 --> ing19_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing19_Pyridoxine -.-> CHEBI_16709
    ing20_Folic_acid["Folic acid"]
    CultureMech_000959 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Riboflavin["Riboflavin"]
    CultureMech_000959 --> ing21_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing21_Riboflavin -.-> CHEBI_17015
    ing22_B12["B12"]
    CultureMech_000959 --> ing22_B12

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 Na2CO3
CO3.2Na 105.988 g/mol
5 G_PER_L CHEBI:29377
sodium carbonate
2 NaCl
Cl.Na 58.443 g/mol
10 G_PER_L CHEBI:26710
sodium chloride
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 Yeast extract 0.02 G_PER_L
5 Peptone 2 G_PER_L
6 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 ZnSO4
O4S.Zn 161.453 g/mol
0.1 G_PER_L CHEBI:35176
zinc sulfate
9 MnCl2
Cl2Mn 125.844 g/mol
0.03 G_PER_L CHEBI:63041
manganese(II) chloride
10 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 CuCl2 x 5 H2O
Cl2Cu.5H2O 224.527 g/mol
0.01 G_PER_L CHEBI:91245
copper(II) chloride pentahydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.02 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 Thiamin
C12H17N4OS 265.362 g/mol
0.0125 G_PER_L CHEBI:18385
thiamine(1+)
16 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0125 G_PER_L CHEBI:31345
Calcium pantothenate
17 Biotin
C10H16N2O3S 244.316 g/mol
0.005 G_PER_L CHEBI:15956
biotin
18 PABA
C7H7NO2 137.138 g/mol
0.0125 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.0125 G_PER_L CHEBI:15940
nicotinic acid
20 Pyridoxine
C8H11NO3 169.18 g/mol
0.025 G_PER_L CHEBI:16709
pyridoxine
21 Folic acid
C19H19N7O6 441.404 g/mol
0.005 G_PER_L CHEBI:27470
folic acid
22 Riboflavin
C17H20N4O6 376.369 g/mol
0.0125 G_PER_L CHEBI:17015
riboflavin
23 B12 0.00125 G_PER_L

Preparation

  1. pH 10.0 - 10.1
  2. Prepare the liquid medium. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
  3. Adjust pH to 3.0-4.0 with HCl.
  4. Autoclave at 121 °C for 15 min.
  5. Note: autoclave in closed bottles
  6. 1: acidic solution (HCl, pH3; 100ml)
  7. 2: basic solution (100ml 0.1N NaOH)
  8. 3: Neutral solution in 100 ml demi water
  9. 4: neutral solution in 100 ml demi water
  10. Sterilize by filtration.
  11. Mix 1:1:1:1 just after addition or add separatlely, each 1ml/L.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.595054Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1491
2026-02-06T05:10:53.775084Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:18:59.924278Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000959
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.490115+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CYCLONATRONUM PROTEIVORANS MEDIUM (OMEGA)" to "cyclonatronum_proteivorans_medium_omega" for consistent matching
2026-05-20T06:01:12.748906+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '10-10.1' -> {'min': 10.0, 'max': 10.1}
2026-06-05T07:40:55.162921+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.084149+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:29.501142+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint e84227fc87b25a51ed44fc65eafd63454a81a95c404af2f71edc531056751f92