pe_medium

CultureMech:000966 bacterial COMPLEX SOLID_AGAR
Original name: PE MEDIUM

Overview

External ontology
mediadive.medium:1498 (source link unavailable) — PE MEDIUM
Applications
  • Microbial cultivation
pH
7.5
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1498.pdf

Composition

flowchart LR
    CultureMech_000966["pe_medium"]
    ing0_Na_glutamate["Na glutamate"]
    CultureMech_000966 --> ing0_Na_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing0_Na_glutamate -.-> CHEBI_64220
    ing1_Sodium_succinate["Sodium succinate"]
    CultureMech_000966 --> ing1_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing1_Sodium_succinate -.-> CHEBI_63675
    ing2_Sodium_acetate["Sodium acetate"]
    CultureMech_000966 --> ing2_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing2_Sodium_acetate -.-> CHEBI_32954
    ing3_Sodium_Thiosulfate["Sodium Thiosulfate"]
    CultureMech_000966 --> ing3_Sodium_Thiosulfate
    CHEBI_132112["CHEBI:132112"]
    ing3_Sodium_Thiosulfate -.-> CHEBI_132112
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_000966 --> ing4_Yeast_extract
    ing5_Casamino_acids["Casamino acids"]
    CultureMech_000966 --> ing5_Casamino_acids
    ing6__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000966 --> ing6__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing6__NH4_2SO4 -.-> CHEBI_62946
    ing7_Agar["Agar"]
    CultureMech_000966 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Na3_EDTA["Na3-EDTA"]
    CultureMech_000966 --> ing8_Na3_EDTA
    CHEBI_63125["CHEBI:63125"]
    ing8_Na3_EDTA -.-> CHEBI_63125
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000966 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000966 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000966 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_NaCl["NaCl"]
    CultureMech_000966 --> ing12_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing12_NaCl -.-> CHEBI_26710
    ing13_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_000966 --> ing13_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing13_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000966 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000966 --> ing15_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing15_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing16_CuSO4_x_6_H2O["CuSO4 x 6 H2O"]
    CultureMech_000966 --> ing16_CuSO4_x_6_H2O
    CHEBI_91246["CHEBI:91246"]
    ing16_CuSO4_x_6_H2O -.-> CHEBI_91246
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000966 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_H3BO3["H3BO3"]
    CultureMech_000966 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_KH2PO4["KH2PO4"]
    CultureMech_000966 --> ing19_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing19_KH2PO4 -.-> CHEBI_63036
    ing20_K2HPO4["K2HPO4"]
    CultureMech_000966 --> ing20_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing20_K2HPO4 -.-> CHEBI_131527
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000966 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_000966 --> ing22_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing22_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing23_Biotin["Biotin"]
    CultureMech_000966 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000966 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25_Vitamin_B12["Vitamin B12"]
    CultureMech_000966 --> ing25_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing25_Vitamin_B12 -.-> CHEBI_176843
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000966 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000966 --> ing27_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing28_Folic_acid["Folic acid"]
    CultureMech_000966 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 Na glutamate
C5H8NO4.Na 169.112 g/mol
0.5 G_PER_L CHEBI:64220
monosodium glutamate
2 Sodium succinate
C4H4O4.2Na 162.052 g/mol
0.5 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
3 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.5 G_PER_L CHEBI:32954
sodium acetate
4 Sodium Thiosulfate
2Na.O3S2 158.111 g/mol
0.5 G_PER_L CHEBI:132112
sodium thiosulfate
5 Yeast extract 0.5 G_PER_L
6 Casamino acids 0.5 G_PER_L
7 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
8 Agar 15 G_PER_L CHEBI:2509
agar
9 Na3-EDTA
C10H13N2O8.3Na 358.19 g/mol
6.532 G_PER_L CHEBI:63125
EDTA trisodium salt
 [Merged 2 duplicates: 4.532, 2.0]
10 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.11 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
11 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
24.65 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
12 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.94 G_PER_L CHEBI:86158
calcium chloride dihydrate
13 NaCl
Cl.Na 58.443 g/mol
23.4 G_PER_L CHEBI:26710
sodium chloride
14 MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
0.1116 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.0288 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0292 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
17 CuSO4 x 6 H2O
Cu.6H2O.O4S 267.699 g/mol
0.0252 G_PER_L CHEBI:91246
copper(II) sulfate hexahydrate
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0242 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 H3BO3
H3BO3 61.833 g/mol
0.031 G_PER_L CHEBI:33118
boric acid
20 KH2PO4
H2O4P.K 136.084 g/mol
136.09 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
21 K2HPO4
HO4P.2K 174.174 g/mol
228.23 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
22 Nicotinic acid
C6H5NO2 123.111 g/mol
1 G_PER_L CHEBI:15940
nicotinic acid
23 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
1 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.05 G_PER_L CHEBI:15956
biotin
25 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.5 G_PER_L CHEBI:30753
4-aminobenzoic acid
26 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12
27 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.5 G_PER_L CHEBI:31345
Calcium pantothenate
28 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.5 G_PER_L CHEBI:30961
pyridoxine hydrochloride
29 Folic acid
C19H19N7O6 441.404 g/mol
0.5 G_PER_L CHEBI:27470
folic acid

Preparation

  1. pH 7.5
  2. Autoclave and add 1ml/l Vitamin solution (see Medium 87A)
  3. Adjust to pH 7.0 with 10% KOH.
  4. pH 7.2
  5. Adjust pH to 7.5. The solution is filter-sterilized.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.627213Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1498
2026-01-28T19:48:22.527502Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:07.274745Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:00.023604Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000966
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.597232+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PE MEDIUM" to "pe_medium" for consistent matching
2026-06-05T07:41:41.022618+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.567388+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:09.917130+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:52.362852+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a4e2bbece4c85ed4fe09c0c43a24383ab3e070091f9089ac41f70779c65bfda6