methylocaldum_marinum_medium

Overview

External ontology

mediadive.medium:1501 (source link unavailable) — METHYLOCALDUM MARINUM MEDIUM

Applications

• Microbial cultivation

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1501.pdf

Composition

flowchart LR
    CultureMech_000971["methylocaldum_marinum_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000971 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Agar["Agar"]
    CultureMech_000971 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509
    ing2_Na2_EDTA["Na2-EDTA"]
    CultureMech_000971 --> ing2_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing2_Na2_EDTA -.-> CHEBI_64734
    ing3_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000971 --> ing3_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing3_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000971 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000971 --> ing5_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing6_H3BO3["H3BO3"]
    CultureMech_000971 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000971 --> ing7_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000971 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000971 --> ing9_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing10_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000971 --> ing10_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing10_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing11_KH2PO4["KH2PO4"]
    CultureMech_000971 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12_KNO3["KNO3"]
    CultureMech_000971 --> ing12_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing12_KNO3 -.-> CHEBI_63043
    ing13_MgSO4_x_6_H2O["MgSO4 x 6 H2O"]
    CultureMech_000971 --> ing13_MgSO4_x_6_H2O
    CHEBI_32599["CHEBI:32599"]
    ing13_MgSO4_x_6_H2O -.-> CHEBI_32599
    ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000971 --> ing14_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing15_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_000971 --> ing15_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing15_Fe_III__EDTA -.-> CHEBI_30729
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000971 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

Preparation

1. Prepare the growth medium as follows:
2. Dilute 100 ml solution 1 to 1 litre with 2% NaCl and then add 1 ml of solution 3, 1 ml of the trace elements, and 0.1 ml of solution 2. If solid media is required add 1.5% agar. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 25% methane to the gas phase and autoclave at 121C (15psi) for 15 minutes. Autoclave the phosphate buffer separately. When the growth medium is cool 10 ml/l of the phosphate buffer is added - if too warm the phosphate will precipitate. Liquid cultures should be grown with shaking.
3. May be stored at 4°C in the dark
4. Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre.
5. Dissolve the ingredients listed above (in that order) in about 700 ml of 2% NaCl, and then make up to 1 litre with 2% NaCl.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• methylocaldum_marinum_medium (CultureMech:000971)