methylobacter_medium_m10_xcf

CultureMech:000972 bacterial DEFINED LIQUID
Original name: METHYLOBACTER MEDIUM M10 XCF

Overview

External ontology
mediadive.medium:1502 (source link unavailable) — METHYLOBACTER MEDIUM M10 XCF
Applications
  • Microbial cultivation
pH range
{'min': 8.0, 'max': 8.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1502.pdf

Composition

flowchart LR
    CultureMech_000972["methylobacter_medium_m10_xcf"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000972 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000972 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000972 --> ing2_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing3_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_000972 --> ing3_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing3_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000972 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_000972 --> ing5_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing5_Fe_III__EDTA -.-> CHEBI_30729
    ing6_CuSO4["CuSO4"]
    CultureMech_000972 --> ing6_CuSO4
    CHEBI_23414["CHEBI:23414"]
    ing6_CuSO4 -.-> CHEBI_23414
    ing7_NaHCO3["NaHCO3"]
    CultureMech_000972 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000972 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000972 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_MnSO4_x_2_H2O["MnSO4 x 2 H2O"]
    CultureMech_000972 --> ing10_MnSO4_x_2_H2O
    CHEBI_86356["CHEBI:86356"]
    ing10_MnSO4_x_2_H2O -.-> CHEBI_86356
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000972 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_H3BO3["H3BO3"]
    CultureMech_000972 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000972 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
3 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
0.5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
4 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.01 G_PER_L CHEBI:91243
calcium chloride hexahydrate
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.005 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
0.05 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
7 CuSO4
Cu.O4S 159.609 g/mol
0.02 G_PER_L CHEBI:23414
copper(II) sulfate
8 NaHCO3
CHO3.Na 84.006 g/mol
9.45 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.44 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 MnSO4 x 2 H2O
2H2O.Mn.O4S 187.031 g/mol
0.17 G_PER_L CHEBI:86356
manganese(II) sulfate dihydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.06 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.08 G_PER_L CHEBI:53503
cobalt chloride hexahydrate

Preparation

  1. Adjust the pH with 1M NaOH or H2SO4 to 8.0-8.4 (after autoclaving and mixing all solutions the pH should be 8.0-8.4)
  2. Dispense the medium into the culture vessels. For small volumes serum bottles or Hungate tubes may be used. The gas phase to liquid ratio should be about 1:10. Use a gas phase containing air, 10-20% methane and 5-10% CO2 and autoclave at 121C for 15 minutes. When the growth medium is cool the NaHCO3 is added. Liquid cultures should be grown with shaking.
  3. Add to the sterile medium per liter from a 8% filter sterilised stock solution:

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.641141Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1502
2026-03-13T06:19:00.077557Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000972
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.656325+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOBACTER MEDIUM M10 XCF" to "methylobacter_medium_m10_xcf" for consistent matching
2026-05-20T06:01:56.690452+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '8-8.4' -> {'min': 8.0, 'max': 8.4}
2026-06-05T07:41:32.995143+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.506937+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.605484+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f89823b091639266e3d23b61a7768692ec4f04a4a5addee307e09efcc43ac2f4