plantomycetaceae_medium_sbp2

CultureMech:000983 bacterial DEFINED LIQUID
Original name: PLANTOMYCETACEAE MEDIUM SBP2

Overview

External ontology
mediadive.medium:1511 (source link unavailable) — PLANTOMYCETACEAE MEDIUM SBP2
Applications
  • Microbial cultivation
pH range
{'min': 7.6, 'max': 8.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1511.pdf

Composition

flowchart LR
    CultureMech_000983["plantomycetaceae_medium_sbp2"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000983 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_000983 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgCl2["MgCl2"]
    CultureMech_000983 --> ing2_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing2_MgCl2 -.-> CHEBI_6636
    ing3_CaCl2["CaCl2"]
    CultureMech_000983 --> ing3_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing3_CaCl2 -.-> CHEBI_3312
    ing4_KCl["KCl"]
    CultureMech_000983 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_KNO3["KNO3"]
    CultureMech_000983 --> ing5_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing5_KNO3 -.-> CHEBI_63043
    ing6_Glucose["Glucose"]
    CultureMech_000983 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_NaHCO3["NaHCO3"]
    CultureMech_000983 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Biotin["Biotin"]
    CultureMech_000983 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Folic_acid["Folic acid"]
    CultureMech_000983 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000983 --> ing10_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing11_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000983 --> ing11_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_HCl -.-> CHEBI_49105
    ing12_Riboflavin["Riboflavin"]
    CultureMech_000983 --> ing12_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing12_Riboflavin -.-> CHEBI_17015
    ing13_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000983 --> ing13_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing13_Nicotinic_acid -.-> CHEBI_15940
    ing14_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000983 --> ing14_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing14_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing15_Vitamin_B12["Vitamin B12"]
    CultureMech_000983 --> ing15_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing15_Vitamin_B12 -.-> CHEBI_176843
    ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000983 --> ing16_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing17__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000983 --> ing17__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing17__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing18_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_000983 --> ing18_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing18_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing19_NiCl2_x_2_H2O["NiCl2 x 2 H2O"]
    CultureMech_000983 --> ing19_NiCl2_x_2_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_2_H2O -.-> CHEBI_34887
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000983 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000983 --> ing21_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing21_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000983 --> ing22_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing23_Na2SeO4["Na2SeO4"]
    CultureMech_000983 --> ing23_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing23_Na2SeO4 -.-> CHEBI_77775
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000983 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000983 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing26_H3BO3["H3BO3"]
    CultureMech_000983 --> ing26_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing26_H3BO3 -.-> CHEBI_33118
    ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000983 --> ing27_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing27_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.33 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.33 G_PER_L CHEBI:31206
ammonium chloride
3 MgCl2
2Cl.Mg 95.211 g/mol
0.0012 G_PER_L CHEBI:6636
magnesium dichloride
4 CaCl2
Ca.2Cl 110.984 g/mol
0.0012 G_PER_L CHEBI:3312
calcium dichloride
5 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
6 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
7 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
8 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
10 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
11 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
12 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
13 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
14 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
15 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
16 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
17 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
18 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
19 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.784 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
20 NiCl2 x 2 H2O
Cl2Ni 129.599 g/mol
0.284 G_PER_L CHEBI:34887
nickel dichloride
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.038 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.099 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
23 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
24 Na2SeO4
2Na.O4Se 188.936 g/mol
0.095 G_PER_L CHEBI:77775
sodium selenate
25 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.033 G_PER_L CHEBI:63939
sodium tungstate dihydrate
26 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
27 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
28 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate

Preparation

  1. Prepare the liquid medium. Dispense the medium into culture vessels. Sterilize by autoclaving. Before inoculation add from sterile stock solutions:
  2. The 8% NaHCO3 solution may be either filter sterilized or autoclaved. When the solution is autoclaved use tightly closed vessels that are filled to a maximum of 50% of the total volume.
  3. The final pH of the medium should be adjusted to pH 7.6-8.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.663161Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1511
2026-01-28T19:48:16.642137Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-06T05:10:23.911738Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:00.152732Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000983
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.743971+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PLANTOMYCETACEAE MEDIUM SBP2" to "plantomycetaceae_medium_sbp2" for consistent matching
2026-05-20T06:02:02.724847+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.6-8' -> {'min': 7.6, 'max': 8.0}
2026-06-05T07:41:42.229084+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.752120+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:10.313763+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.283571+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 69240cc9bec778249d4d795142aa8b7dad03f946db20a40c74a2ff94d01c1951