sulfurimonas_gotlandica_medium

CultureMech:000985 bacterial DEFINED LIQUID
Original name: SULFURIMONAS GOTLANDICA MEDIUM

Overview

External ontology
mediadive.medium:1513 (source link unavailable) — SULFURIMONAS GOTLANDICA MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.3, 'max': 7.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1513.pdf

Composition

flowchart LR
    CultureMech_000985["sulfurimonas_gotlandica_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000985 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000985 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000985 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KCl["KCl"]
    CultureMech_000985 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Na2SO4["Na2SO4"]
    CultureMech_000985 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_KNO3["KNO3"]
    CultureMech_000985 --> ing5_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing5_KNO3 -.-> CHEBI_63043
    ing6_HEPES_buffer["HEPES buffer"]
    CultureMech_000985 --> ing6_HEPES_buffer
    CHEBI_46756["CHEBI:46756"]
    ing6_HEPES_buffer -.-> CHEBI_46756
    ing7_NH4Cl["NH4Cl"]
    CultureMech_000985 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_SrCl2["SrCl2"]
    CultureMech_000985 --> ing8_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing8_SrCl2 -.-> CHEBI_36383
    ing9_H3BO3["H3BO3"]
    CultureMech_000985 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_NaF["NaF"]
    CultureMech_000985 --> ing10_NaF
    CHEBI_28741["CHEBI:28741"]
    ing10_NaF -.-> CHEBI_28741
    ing11_K2HPO4["K2HPO4"]
    CultureMech_000985 --> ing11_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing11_K2HPO4 -.-> CHEBI_131527
    ing12_NaHCO3["NaHCO3"]
    CultureMech_000985 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000985 --> ing13_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing13_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing14_HCl["HCl"]
    CultureMech_000985 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000985 --> ing15_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing16_ZnCl2["ZnCl2"]
    CultureMech_000985 --> ing16_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing16_ZnCl2 -.-> CHEBI_49976
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000985 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000985 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000985 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000985 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000985 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_NaOH["NaOH"]
    CultureMech_000985 --> ing22_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing22_NaOH -.-> CHEBI_32145
    ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_000985 --> ing23_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000985 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing25_Biotin["Biotin"]
    CultureMech_000985 --> ing25_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_Biotin -.-> CHEBI_15956
    ing26_Folic_acid["Folic acid"]
    CultureMech_000985 --> ing26_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing26_Folic_acid -.-> CHEBI_27470
    ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_000985 --> ing27_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing28_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000985 --> ing28_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing28_Thiamine_HCl -.-> CHEBI_49105
    ing29_Riboflavin["Riboflavin"]
    CultureMech_000985 --> ing29_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing29_Riboflavin -.-> CHEBI_17015
    CultureMech_000985 --> more["...5 more"]

Ingredients (35)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
5.53892 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.28543 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.339321 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 KCl
Cl.K 74.551 g/mol
0.149701 G_PER_L CHEBI:32588
potassium chloride
5 Na2SO4
2Na.O4S 142.043 g/mol
0.908184 G_PER_L CHEBI:32149
sodium sulfate
6 KNO3
K.NO3 101.102 g/mol
1.00798 G_PER_L CHEBI:63043
potassium nitrate
7 HEPES buffer 2.37525 G_PER_L CHEBI:46756
HEPES
8 NH4Cl
Cl.H4N 53.492 g/mol
0.0499002 G_PER_L CHEBI:31206
ammonium chloride
9 SrCl2
2Cl.Sr 158.526 g/mol
0.00798403 G_PER_L CHEBI:36383
strontium dichloride
10 H3BO3
H3BO3 61.833 g/mol
0.01198802 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.00598802, 0.006]
11 NaF
F.Na 41.988 g/mol
0.00399202 G_PER_L CHEBI:28741
sodium fluoride
12 K2HPO4
HO4P.2K 174.174 g/mol
0.000998004 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
13 NaHCO3
CHO3.Na 84.006 g/mol
0.199601 G_PER_L CHEBI:32139
sodium hydrogencarbonate
14 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2.47505 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
15 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
16 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
17 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 NaOH
HO.Na 39.997 g/mol
0.5 G_PER_L CHEBI:32145
sodium hydroxide
24 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
25 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
26 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
27 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
28 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
29 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
30 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
31 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
32 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
33 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
34 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
35 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients (except bicarbonate, thiosulfate and vitamins) and adjust pH to 7.3. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic serum vials (e.g., Balch-type tubes) and autoclave. Add thiosulfate and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and thiosulftae should be sterilized by filtration. Adjust pH of the complete medium to 7.3 - 7.5, if necessary.
  2. After inoculation pressurize vials to 0.7 bar overpressure with sterile 100% N2 gas.
  3. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:14.670472Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1513
2026-02-06T05:10:52.127823Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:39.433438Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:00.176681Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000985
2026-03-17T06:30:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:11.768817+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFURIMONAS GOTLANDICA MEDIUM" to "sulfurimonas_gotlandica_medium" for consistent matching
2026-05-20T06:02:06.639897+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5}
2026-06-05T07:41:47.753173+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.881284+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:12.149849+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:57.014348+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6c6d7c461afb39f094ebf25c415d62690776333936df676e6c9dbc71a08e9587