Overview
- External ontology
- mediadive.medium:1524 (source link unavailable) — PLANCTOMYCETES MEDIUM NH11
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1524.pdf
Composition
flowchart LR
CultureMech_000995["planctomycetes_medium_nh11"]
ing0_Peptone["Peptone"]
CultureMech_000995 --> ing0_Peptone
ing1_Glucose["Glucose"]
CultureMech_000995 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_HEPES["HEPES"]
CultureMech_000995 --> ing2_HEPES
CHEBI_46756["CHEBI:46756"]
ing2_HEPES -.-> CHEBI_46756
ing3_Agar["Agar"]
CultureMech_000995 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000995 --> ing4_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000995 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000995 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000995 --> ing7_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000995 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_Biotin["Biotin"]
CultureMech_000995 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Folic_acid["Folic acid"]
CultureMech_000995 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000995 --> ing11_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing12_Riboflavine["Riboflavine"]
CultureMech_000995 --> ing12_Riboflavine
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavine -.-> CHEBI_17015
ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_000995 --> ing13_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing14_Nicotinamide["Nicotinamide"]
CultureMech_000995 --> ing14_Nicotinamide
CHEBI_17154["CHEBI:17154"]
ing14_Nicotinamide -.-> CHEBI_17154
ing15_D_Calcium_pantothenate["D-Calcium pantothenate"]
CultureMech_000995 --> ing15_D_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_D_Calcium_pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_000995 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_000995 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_NaCl["NaCl"]
CultureMech_000995 --> ing18_NaCl
CHEBI_26710["CHEBI:26710"]
ing18_NaCl -.-> CHEBI_26710
ing19_Na2SO4["Na2SO4"]
CultureMech_000995 --> ing19_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing19_Na2SO4 -.-> CHEBI_32149
ing20_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000995 --> ing20_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing20_MgCl2_x_6_H2O -.-> CHEBI_86345
ing21_CaCl2["CaCl2"]
CultureMech_000995 --> ing21_CaCl2
CHEBI_3312["CHEBI:3312"]
ing21_CaCl2 -.-> CHEBI_3312
ing22_NaHCO3["NaHCO3"]
CultureMech_000995 --> ing22_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing22_NaHCO3 -.-> CHEBI_32139
ing23_KCl["KCl"]
CultureMech_000995 --> ing23_KCl
CHEBI_32588["CHEBI:32588"]
ing23_KCl -.-> CHEBI_32588
ing24_KBr["KBr"]
CultureMech_000995 --> ing24_KBr
CHEBI_32030["CHEBI:32030"]
ing24_KBr -.-> CHEBI_32030
ing25_H3BO3["H3BO3"]
CultureMech_000995 --> ing25_H3BO3
CHEBI_33118["CHEBI:33118"]
ing25_H3BO3 -.-> CHEBI_33118
ing26_SrCl2["SrCl2"]
CultureMech_000995 --> ing26_SrCl2
CHEBI_36383["CHEBI:36383"]
ing26_SrCl2 -.-> CHEBI_36383
ing27_NaF["NaF"]
CultureMech_000995 --> ing27_NaF
CHEBI_28741["CHEBI:28741"]
ing27_NaF -.-> CHEBI_28741
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.33557 G_PER_L | ||
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
0.167785 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 | HEPES | 1.59732 G_PER_L |
CHEBI:46756
HEPES
|
|
| 4 | Agar | 10.0671 G_PER_L |
CHEBI:2509
agar
|
|
| 5 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10.5263 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
31.2632 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.51579 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0133368 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.104211 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.004 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.004 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.02 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 13 |
Riboflavine
C17H20N4O6 376.369 g/mol
|
0.01 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.01 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 15 |
Nicotinamide
C6H6N2O 122.127 g/mol
|
0.01 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 16 |
D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.01 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0002 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.01 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
NaCl
Cl.Na 58.443 g/mol
|
23.47 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 20 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.92 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 21 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.98 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 22 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 23 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.192 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 24 |
KCl
Cl.K 74.551 g/mol
|
0.664 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 25 |
KBr
Br.K 119.002 g/mol
|
0.096 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 26 |
H3BO3
H3BO3 61.833 g/mol
|
0.026 G_PER_L |
CHEBI:33118
boric acid
|
|
| 27 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.024 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 28 |
NaF
F.Na 41.988 g/mol
|
0.003 G_PER_L |
CHEBI:28741
sodium fluoride
|
Preparation
- Sterilize 20 min/121°C. After cooling to 60°C add to the medium:
- Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
- Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.
- Store in the dark and cold (5°C).
Solutions
Metall salt sol. 44
Dose: 50 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- planctomycetes_medium_nh11 (CultureMech:000995)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.703604Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1524 |
| 2026-01-28T19:48:14.898995Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:24.781003+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:00.263519Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000995 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:11.863091+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PLANCTOMYCETES MEDIUM NH11" to "planctomycetes_medium_nh11" for consistent matching |
| 2026-05-16T09:04:29.235940+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:42.197279+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.301365+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.247626+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8efe049a58c85be4c66986115b68ef4c087968252e4c4937e8a2ffe168caa7e0 |