salinivirga_l21_hs_medium

CultureMech:001004 bacterial COMPLEX LIQUID
Original name: SALINIVIRGA (L21 HS) MEDIUM

Overview

External ontology
mediadive.medium:1527a (source link unavailable) — SALINIVIRGA (L21 HS) MEDIUM
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'salinispira_l21_ls_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1526', 'original_category': 'bacterial'}
pH range
{'min': 7.3, 'max': 7.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1527a.pdf

Composition

flowchart LR
    CultureMech_001004["salinivirga_l21_hs_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_001004 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001004 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_001004 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_Na2SO4["Na2SO4"]
    CultureMech_001004 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_NH4Cl["NH4Cl"]
    CultureMech_001004 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001004 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_K2HPO4["K2HPO4"]
    CultureMech_001004 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001004 --> ing7_Yeast_extract
    ing8_Sodium_resazurin["Sodium resazurin"]
    CultureMech_001004 --> ing8_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Sodium_resazurin -.-> CHEBI_8806
    ing9_Na2CO3["Na2CO3"]
    CultureMech_001004 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377
    ing10_Trypticase_peptone["Trypticase peptone"]
    CultureMech_001004 --> ing10_Trypticase_peptone
    ing11_D_Glucose["D-Glucose"]
    CultureMech_001004 --> ing11_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing11_D_Glucose -.-> CHEBI_17634
    ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_001004 --> ing12_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001004 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001004 --> ing14_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001004 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001004 --> ing16_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing16_MnSO4_x_H2O -.-> CHEBI_86364
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001004 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_001004 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001004 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001004 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_001004 --> ing21_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing21_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing22_H3BO3["H3BO3"]
    CultureMech_001004 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001004 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001004 --> ing24_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_001004 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing26_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_001004 --> ing26_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing26_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing27_Biotin["Biotin"]
    CultureMech_001004 --> ing27_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing27_Biotin -.-> CHEBI_15956
    ing28_Folic_acid["Folic acid"]
    CultureMech_001004 --> ing28_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing28_Folic_acid -.-> CHEBI_27470
    ing29_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001004 --> ing29_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing29_Pyridoxine_hydrochloride -.-> CHEBI_30961
    CultureMech_001004 --> more["...7 more"]

Ingredients (37)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
60.3472 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 59.3472, 1.0]
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.93472 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KCl
Cl.K 74.551 g/mol
1.48368 G_PER_L CHEBI:32588
potassium chloride
4 Na2SO4
2Na.O4S 142.043 g/mol
0.98912 G_PER_L CHEBI:32149
sodium sulfate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.98912 G_PER_L CHEBI:31206
ammonium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.495648 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.395648, 0.1]
7 K2HPO4
HO4P.2K 174.174 g/mol
0.395648 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 Yeast extract 0.98912 G_PER_L
9 Sodium resazurin
C12H7NO4 229.191 g/mol
0.00049456 G_PER_L CHEBI:8806
Resazurin
10 Na2CO3
CO3.2Na 105.988 g/mol
1.48368 G_PER_L CHEBI:29377
sodium carbonate
11 Trypticase peptone 0.98912 G_PER_L
12 D-Glucose
C6H12O6 180.156 g/mol
0.98912 G_PER_L CHEBI:17634
D-glucose
13 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.49456 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.49456 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
16 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
17 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.5 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
19 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.18 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
21 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
22 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.02 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
23 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0003 G_PER_L CHEBI:131361
disodium selenite pentahydrate
27 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0004 G_PER_L CHEBI:63939
sodium tungstate dihydrate
28 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
29 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
30 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.1 G_PER_L CHEBI:30961
pyridoxine hydrochloride
31 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
32 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
33 Nicotinic acid
C6H5NO2 123.111 g/mol
0.05 G_PER_L CHEBI:15940
nicotinic acid
34 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
35 Vitamin B12 0.001 G_PER_L CHEBI:176843
vitamin B12
36 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
37 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Dissolve ingredients except carbonate, peptone, glucose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add peptone, glucose, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary.
  2. Note: Use at least 5-10% (v/v) inoculum.
  3. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-26T17:53:14.748333Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1527a
2026-02-06T05:10:30.852907Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:06.883576Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:00.406216Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001004
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.018041+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SALINIVIRGA (L21 HS) MEDIUM" to "salinivirga_l21_hs_medium" for consistent matching
2026-05-20T06:02:04.734904+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.3-7.5' -> {'min': 7.3, 'max': 7.5}
2026-06-05T07:41:45.145437+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.266381+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:55.170329+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: salinispira_l21_ls_medium, salinivirga_l21_hs_medium
2026-08-06T03:05:55.170374+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 60105efb985d9906a5f759d85800093d95dbb0ff65663397708147b833bea91d