modified_tryptone_soy_broth_agar

CultureMech:001008 bacterial COMPLEX SOLID_AGAR
Original name: MODIFIED TRYPTONE SOY BROTH/AGAR

Overview

External ontology
mediadive.medium:1530 (source link unavailable) — MODIFIED TRYPTONE SOY BROTH/AGAR
Applications
  • Microbial cultivation
pH
10.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1530.pdf

Composition

flowchart LR
    CultureMech_001008["modified_tryptone_soy_broth_agar"]
    ing0_Casein_peptone["Casein peptone"]
    CultureMech_001008 --> ing0_Casein_peptone
    ing1_Soy_peptone["Soy peptone"]
    CultureMech_001008 --> ing1_Soy_peptone
    ing2_D____Glucose["D(+)-Glucose"]
    CultureMech_001008 --> ing2_D____Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_D____Glucose -.-> CHEBI_17634
    ing3_NaCl["NaCl"]
    CultureMech_001008 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_K2HPO4["K2HPO4"]
    CultureMech_001008 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Na2CO3["Na2CO3"]
    CultureMech_001008 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Agar["Agar"]
    CultureMech_001008 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Casein peptone 17 G_PER_L
2 Soy peptone 3 G_PER_L
3 D(+)-Glucose
C6H12O6 180.156 g/mol
2.5 G_PER_L CHEBI:17634
D-glucose
4 NaCl
Cl.Na 58.443 g/mol
45 G_PER_L CHEBI:26710
sodium chloride
5 K2HPO4
HO4P.2K 174.174 g/mol
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Na2CO3
CO3.2Na 105.988 g/mol
10 G_PER_L CHEBI:29377
sodium carbonate
7 Agar 20 G_PER_L CHEBI:2509
agar
(for solid medium)

Preparation

  1. Adjust pH to 10.5
  2. The basic medium is the same as Caso Bouillon (Merck or Oxoid). The medium may be solidified by adding 20.0 g/l agar.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.760847Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1530
2026-03-13T06:19:00.442378Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001008
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.058591+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED TRYPTONE SOY BROTH/AGAR" to "modified_tryptone_soy_broth_agar" for consistent matching
2026-06-05T07:41:37.953066+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.018669+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:49.976207+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2028ef048b99ec205dc4f459fb800d652baac0406ec05c66d64f63e3cdd1aa6e