planctomyces_pyreformis_medium

CultureMech:001009 bacterial COMPLEX SOLID_AGAR
Original name: PLANCTOMYCES PYREFORMIS MEDIUM

Overview

External ontology
mediadive.medium:1531 (source link unavailable) — PLANCTOMYCES PYREFORMIS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 5.8, 'max': 6.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1531.pdf

Composition

flowchart LR
    CultureMech_001009["planctomyces_pyreformis_medium"]
    ing0_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_001009 --> ing0_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing0_N_Acetylglucosamine -.-> CHEBI_59640
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001009 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_001009 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001009 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001009 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_001009 --> ing5_Yeast_extract
    ing6_Glucose["Glucose"]
    CultureMech_001009 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Agar["Agar"]
    CultureMech_001009 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001009 --> ing8_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing9__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001009 --> ing9__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing9__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001009 --> ing10_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing11_Na_EDTA["Na-EDTA"]
    CultureMech_001009 --> ing11_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing11_Na_EDTA -.-> CHEBI_64734
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001009 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001009 --> ing13_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing13_MnSO4_x_H2O -.-> CHEBI_86364
    ing14_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001009 --> ing14_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing14_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing15_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001009 --> ing15_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing15_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing16_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001009 --> ing16_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing16_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing17_Biotin["Biotin"]
    CultureMech_001009 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_001009 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001009 --> ing19_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing20_Riboflavine["Riboflavine"]
    CultureMech_001009 --> ing20_Riboflavine
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavine -.-> CHEBI_17015
    ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001009 --> ing21_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing22_Nicotinamide["Nicotinamide"]
    CultureMech_001009 --> ing22_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing22_Nicotinamide -.-> CHEBI_17154
    ing23_D_Calcium_pantothenate["D-Calcium pantothenate"]
    CultureMech_001009 --> ing23_D_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_D_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Vitamin_B12["Vitamin B12"]
    CultureMech_001009 --> ing24_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing24_Vitamin_B12 -.-> CHEBI_176843
    ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001009 --> ing25_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing25_p_Aminobenzoic_acid -.-> CHEBI_30753

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 N-Acetylglucosamine 1 G_PER_L CHEBI:59640
N-acetylglucosamine
2 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.2 G_PER_L CHEBI:62946
ammonium sulfate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.8 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.1, 29.7]
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.36 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.02, 3.34]
6 Yeast extract 0.2 G_PER_L
7 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
8 Agar 18 G_PER_L CHEBI:2509
agar
9 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
10 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
11 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
12 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
13 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
14 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
15 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
16 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
17 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
18 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
19 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
20 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
21 Riboflavine
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
22 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
23 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
24 D-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
25 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
26 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid

Preparation

  1. Adjust pH to 5.8 - 6.5.
  2. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
  3. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.
  4. Store in the dark and cold (5°C).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.762599Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1531
2026-01-28T19:48:07.403583Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:08:38.728497Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:00.452314Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001009
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.068858+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PLANCTOMYCES PYREFORMIS MEDIUM" to "planctomyces_pyreformis_medium" for consistent matching
2026-05-20T06:02:02.674525+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5.8-6.5' -> {'min': 5.8, 'max': 6.5}
2026-06-05T07:41:42.164110+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.289450+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.212023+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint b5d346ed29f7289a670e1c5e908d92240c209f5bea5a1ed9fcb1e749f189f054