marinifilum_medium_ird_medium

CultureMech:001011 bacterial COMPLEX LIQUID
Original name: MARINIFILUM MEDIUM (IRD MEDIUM)

Overview

External ontology
mediadive.medium:1533 (source link unavailable) — MARINIFILUM MEDIUM (IRD MEDIUM)
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1533.pdf

Composition

flowchart LR
    CultureMech_001011["marinifilum_medium_ird_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_001011 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_001011 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_001011 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_001011 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_001011 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001011 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Cysteine_HCl["Cysteine HCl"]
    CultureMech_001011 --> ing6_Cysteine_HCl
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_001011 --> ing7_Yeast_extract
    ing8_Resazurin["Resazurin"]
    CultureMech_001011 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001011 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_NaHCO3["NaHCO3"]
    CultureMech_001011 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_001011 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Glucose["Glucose"]
    CultureMech_001011 --> ing12_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing12_Glucose -.-> CHEBI_17234
    ing13_HCl["HCl"]
    CultureMech_001011 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeCl2_x_7_H2O["FeCl2 x 7 H2O"]
    CultureMech_001011 --> ing14_FeCl2_x_7_H2O
    CHEBI_30812["CHEBI:30812"]
    ing14_FeCl2_x_7_H2O -.-> CHEBI_30812
    ing15_ZnCl2["ZnCl2"]
    CultureMech_001011 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_001011 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_001011 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_001011 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_001011 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_001011 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_001011 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.3 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
25 G_PER_L CHEBI:26710
sodium chloride
5 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Cysteine HCl 0.5 G_PER_L
8 Yeast extract 1 G_PER_L
9 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 NaHCO3
CHO3.Na 84.006 g/mol
2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.4 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Glucose
C6H12O6 180.156 g/mol
3.6032 G_PER_L CHEBI:17234
glucose
14 HCl
HCl 36.461 g/mol
1.625 G_PER_L CHEBI:17883
hydrogen chloride
15 FeCl2 x 7 H2O
Cl2Fe 126.751 g/mol
1.5 G_PER_L CHEBI:30812
iron dichloride
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.062 G_PER_L CHEBI:33118
boric acid
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. pH 7.0
  2. Add after autoclave
  3. Prepare the medium without NaHCO3, Na2S x 9 H2O, MgCl2 x 6 H2O and glucose. Boil the medium in a water bath and cool under a stream of N2/CO2 (80/20). Add the NaHCO3 to the cooled medium (the pH should be 7.0) and dispense the medium into Hungate tubes or serum bottles under a stream of N2/CO2 (80/20). Seal the tubes under N2/CO2 (80/20) and autoclave. To the cooled medium at the appropriate amounts of Na2S x 9 H2O, MgCl2 x 6 H2O and glucose from sterile, anaerobic stock solutions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.768903Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1533
2026-03-13T06:19:00.466955Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001011
2026-03-17T06:29:45.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.084972+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MARINIFILUM MEDIUM (IRD MEDIUM)" to "marinifilum_medium_ird_medium" for consistent matching
2026-06-05T07:41:13.101016+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.956005+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:42.146436+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3f177caba94d7df3b147ee333db89bfdb5cad54b9b269a41c098fc0bba462c79