Overview
- External ontology
- mediadive.medium:1535 (source link unavailable) — MEDIUM D (20.6%NACL)
- Applications
-
- Microbial cultivation
- pH
- 7.4
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1535.pdf
Composition
flowchart LR
CultureMech_001013["medium_d_20_6_nacl"]
ing0_NaCl["NaCl"]
CultureMech_001013 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_001013 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001013 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001013 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MnCl2["MnCl2"]
CultureMech_001013 --> ing4_MnCl2
CHEBI_63041["CHEBI:63041"]
ing4_MnCl2 -.-> CHEBI_63041
ing5_Yeast_extract["Yeast extract"]
CultureMech_001013 --> ing5_Yeast_extract
ing6_Agar["Agar"]
CultureMech_001013 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
206 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.37 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
36 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MnCl2
Cl2Mn 125.844 g/mol
|
0.013 G_PER_L |
CHEBI:63041
manganese(II) chloride
|
|
| 6 | Yeast extract | 5 G_PER_L | ||
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
Preparation
- pH 7.4
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- medium_d_20_6_nacl (CultureMech:001013)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.776748Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1535 |
| 2026-02-06T05:10:48.076574Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:00.487337Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001013 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.107807+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM D (20.6%NACL)" to "medium_d_20_6_nacl" for consistent matching |
| 2026-06-05T07:41:28.774849+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:05.944974+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.936142+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8b6f5da40d906e38d456a9a874247542db43ea3273f0846581775e9a574cebe8 |