Overview
- External ontology
- mediadive.medium:1540 (source link unavailable) — BHA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.6, 'max': 8.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1540.pdf
Composition
flowchart LR
CultureMech_001018["bha_medium"]
ing0_Bacto_peptone["Bacto peptone"]
CultureMech_001018 --> ing0_Bacto_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_001018 --> ing1_Yeast_extract
ing2_Tris_HCl["Tris HCl"]
CultureMech_001018 --> ing2_Tris_HCl
CHEBI_9754["CHEBI:9754"]
ing2_Tris_HCl -.-> CHEBI_9754
ing3_NaCl["NaCl"]
CultureMech_001018 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001018 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_KCl["KCl"]
CultureMech_001018 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NH4Cl["NH4Cl"]
CultureMech_001018 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_CaCl2["CaCl2"]
CultureMech_001018 --> ing7_CaCl2
CHEBI_3312["CHEBI:3312"]
ing7_CaCl2 -.-> CHEBI_3312
ing8_Agar["Agar"]
CultureMech_001018 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone | 5 G_PER_L | ||
| 2 | Yeast extract | 5 G_PER_L | ||
| 3 |
Tris HCl
C4H11NO3 121.136 g/mol
|
6 G_PER_L |
CHEBI:9754
tris
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
11.7 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7.84 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.74 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.15 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Final pH should be 7.8 ± 0.2 at 25°C. The medium may be solidified by adding 15.0g/l agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bha_medium (CultureMech:001018)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.791553Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1540 |
| 2026-02-06T05:10:50.843451Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:00.531455Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001018 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.157375+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BHA MEDIUM" to "bha_medium" for consistent matching |
| 2026-05-20T06:01:09.822480+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.6-8' -> {'min': 7.6, 'max': 8.0} |
| 2026-06-05T07:40:50.861163+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:53.893132+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:25.105499+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 51b48e9511143082d093903cd266cc95816f6d9bdee09c196cf48fadf4e14cff |