pirellula_medium_m30

CultureMech:001022 bacterial COMPLEX LIQUID
Original name: . PIRELLULA MEDIUM (M30)

Overview

External ontology
mediadive.medium:1544 (source link unavailable) — . PIRELLULA MEDIUM (M30)
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1544.pdf

Composition

flowchart LR
    CultureMech_001022["pirellula_medium_m30"]
    ing0_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_001022 --> ing0_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing0_Tris_HCl_buffer -.-> CHEBI_9754
    ing1_Gelrite["Gelrite"]
    CultureMech_001022 --> ing1_Gelrite
    CHEBI_85248["CHEBI:85248"]
    ing1_Gelrite -.-> CHEBI_85248
    ing2_MgCl2["MgCl2"]
    CultureMech_001022 --> ing2_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing2_MgCl2 -.-> CHEBI_6636
    ing3_Sea_water["Sea water"]
    CultureMech_001022 --> ing3_Sea_water
    ing4_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_001022 --> ing4_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing4_N_Acetylglucosamine -.-> CHEBI_59640
    ing5_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_001022 --> ing5_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing5_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing6_Vitamin_B12["Vitamin B12"]
    CultureMech_001022 --> ing6_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing6_Vitamin_B12 -.-> CHEBI_176843
    ing7_Biotin["Biotin"]
    CultureMech_001022 --> ing7_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing7_Biotin -.-> CHEBI_15956
    ing8_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_001022 --> ing8_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing8_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing9_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_001022 --> ing9_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing9_Calcium_pantothenate -.-> CHEBI_31345
    ing10_Folic_acid["Folic acid"]
    CultureMech_001022 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Riboflavin["Riboflavin"]
    CultureMech_001022 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Nicotinamide["Nicotinamide"]
    CultureMech_001022 --> ing12_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing12_Nicotinamide -.-> CHEBI_17154
    ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_001022 --> ing13_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_001022 --> ing14_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001022 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001022 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001022 --> ing17_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing17_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing18__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001022 --> ing18__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing18__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001022 --> ing19_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing20_Na_EDTA["Na-EDTA"]
    CultureMech_001022 --> ing20_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing20_Na_EDTA -.-> CHEBI_64734
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001022 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001022 --> ing22_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing22_MnSO4_x_H2O -.-> CHEBI_86364
    ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001022 --> ing23_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing24_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001022 --> ing24_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing24_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing25_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001022 --> ing25_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing25_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 Tris-HCl buffer
C4H11NO3 121.136 g/mol
50 G_PER_L CHEBI:9754
tris
2 Gelrite 8.33333 G_PER_L CHEBI:85248
gellan gum
3 MgCl2
2Cl.Mg 95.211 g/mol
1.04167 G_PER_L CHEBI:6636
magnesium dichloride
4 Sea water 700 G_PER_L
5 N-Acetylglucosamine 40 G_PER_L CHEBI:59640
N-acetylglucosamine
6 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
0.2 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
7 Vitamin B12 0.0002 G_PER_L CHEBI:176843
vitamin B12
8 Biotin
C10H16N2O3S 244.316 g/mol
0.004 G_PER_L CHEBI:15956
biotin
9 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.01 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate
10 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.01 G_PER_L CHEBI:31345
Calcium pantothenate
11 Folic acid
C19H19N7O6 441.404 g/mol
0.004 G_PER_L CHEBI:27470
folic acid
12 Riboflavin
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
13 Nicotinamide
C6H6N2O 122.127 g/mol
0.01 G_PER_L CHEBI:17154
nicotinamide
14 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.01 G_PER_L CHEBI:30753
4-aminobenzoic acid
15 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.02 G_PER_L CHEBI:30961
pyridoxine hydrochloride
16 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
17 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.7 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
18 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.335 G_PER_L CHEBI:86158
calcium chloride dihydrate
19 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
20 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
21 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
24 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
25 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
26 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. Adjust pH to 7.5, autoclave at 121°C for 20 min.
  2. Adjust pH to 7.5, filter-sterilize.
  3. Dissolve the nitrilotriacetic acid.
  4. Adjust pH to 7.0 with KOH (~7.3 g).
  5. Dissolve other salts separately and combine.
  6. Adjust pH to 6.8 with NaOH or H2SO4.
  7. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.798050Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1544
2026-03-13T03:09:27.610385Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:00.552363Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001022
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.180488+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from ". PIRELLULA MEDIUM (M30)" to "pirellula_medium_m30" for consistent matching
2026-06-05T07:41:41.882298+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.223052+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:52.935119+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint aa8fe947d811304660f6a8723869f17246b28119e230114ad4c8f783356216a2