medium_for_tick_cells_rickettsia

Overview

External ontology

mediadive.medium:1555 (source link unavailable) — Medium for tick cells (Rickettsia)

Applications

• Microbial cultivation

pH

7.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1555.pdf

Composition

flowchart LR
    CultureMech_001032["medium_for_tick_cells_rickettsia"]
    ing0_L_Aspartic_acid["L-Aspartic acid"]
    CultureMech_001032 --> ing0_L_Aspartic_acid
    CHEBI_17053["CHEBI:17053"]
    ing0_L_Aspartic_acid -.-> CHEBI_17053
    ing1_L_Glutamine["L-Glutamine"]
    CultureMech_001032 --> ing1_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing1_L_Glutamine -.-> CHEBI_18050
    ing2_L_Proline["L-Proline"]
    CultureMech_001032 --> ing2_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing2_L_Proline -.-> CHEBI_17203
    ing3_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_001032 --> ing3_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing3_L_Glutamic_acid -.-> CHEBI_16015
    ing4_alpha_ketoglutaric_acid["alpha-ketoglutaric acid"]
    CultureMech_001032 --> ing4_alpha_ketoglutaric_acid
    CHEBI_30915["CHEBI:30915"]
    ing4_alpha_ketoglutaric_acid -.-> CHEBI_30915
    ing5_D_Glucose["D-Glucose"]
    CultureMech_001032 --> ing5_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing5_D_Glucose -.-> CHEBI_17634
    ing6_NaOH["NaOH"]
    CultureMech_001032 --> ing6_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing6_NaOH -.-> CHEBI_32145
    ing7_Fetal_bovine_serum["Fetal bovine serum"]
    CultureMech_001032 --> ing7_Fetal_bovine_serum
    ing8_Tryptose_phosphate_broth["Tryptose-phosphate broth"]
    CultureMech_001032 --> ing8_Tryptose_phosphate_broth
    ing9_lipoprotein_concentrate["lipoprotein concentrate"]
    CultureMech_001032 --> ing9_lipoprotein_concentrate
    ing10_HEPES["HEPES"]
    CultureMech_001032 --> ing10_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing10_HEPES -.-> CHEBI_46756
    ing11_NaHCO3["NaHCO3"]
    CultureMech_001032 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139

Ingredients (12)

Preparation

1. Prior to use in supplemented medium, L15C was diluted by the addition of 33 % water by volume.
2. Supplemented L15C medium is used with
3. The pH is adjusted to pH 7.5 using 1 M NaOH.
4. <b>Cultivation of IRE11 cells for infection with Rickettsia monacensis :</b>
5. This medium and the cultivation of tick cells is described in the "Basic Tick Cell Culture Methods" provided as pdf-file (see strain entry for DSM 29017: cultivation conditions) by Tim Kurtti & Uli Munderloh, University of Minnesota.
6. L15C (Modified Leibovitzs' L15) used to culture host cells (IRE11). L15C medium is a modification of L15B medium, previously described in detail (Munderloh UG, Kurtti TJ. 1989. Formulation of medium for tick cell culture. Exp. Appl. Acarol. 7:219-29).
7. Alterations were made to the following reagents:
8. <b>Infection with Rickettsia monacensis or R. buchneri :</b> One 25 cm2 flask of well-grown cells is infected with 1 ml of the Rickettsia strain (quickly thawed from -80°C ) and centrifuged for 1 h onto the cell layer at 1600 rpm at 20°C . Incubate at 30°C with closed lid (without CO2) and look for bacteria and destroyed cells after 4-6 days .

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• medium_for_tick_cells_rickettsia (CultureMech:001032)