paludisphaera_borealis_medium_px4

CultureMech:001034 bacterial COMPLEX SOLID_AGAR
Original name: PALUDISPHAERA BOREALIS MEDIUM (PX4)

Overview

External ontology
mediadive.medium:1557 (source link unavailable) — PALUDISPHAERA BOREALIS MEDIUM (PX4)
Applications
  • Microbial cultivation
pH range
{'min': 5.8, 'max': 6.5}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1557.pdf

Composition

flowchart LR
    CultureMech_001034["paludisphaera_borealis_medium_px4"]
    ing0_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_001034 --> ing0_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing0_N_Acetylglucosamine -.-> CHEBI_59640
    ing1_KH2PO4["KH2PO4"]
    CultureMech_001034 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001034 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001034 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Na_ampicillin["Na-ampicillin"]
    CultureMech_001034 --> ing4_Na_ampicillin
    CHEBI_34535["CHEBI:34535"]
    ing4_Na_ampicillin -.-> CHEBI_34535
    ing5_Peptone["Peptone"]
    CultureMech_001034 --> ing5_Peptone
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_001034 --> ing6_Yeast_extract
    ing7_Glucose["Glucose"]
    CultureMech_001034 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Agar["Agar"]
    CultureMech_001034 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001034 --> ing9_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing10__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_001034 --> ing10__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing10__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001034 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_Na_EDTA["Na-EDTA"]
    CultureMech_001034 --> ing12_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing12_Na_EDTA -.-> CHEBI_64734
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001034 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing14_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001034 --> ing14_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing14_MnSO4_x_H2O -.-> CHEBI_86364
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001034 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001034 --> ing16_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing16_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing17_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001034 --> ing17_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing17_Na2B4O7_x_10_H2O -.-> CHEBI_131366

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 N-Acetylglucosamine 1 G_PER_L CHEBI:59640
N-acetylglucosamine
2 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
29.75 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 0.05, 29.7]
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
3.3499999999999996 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.01, 3.34]
5 Na-ampicillin
C16H18N3O4S.Na 371.394 g/mol
0.2 G_PER_L CHEBI:34535
ampicillin sodium
6 Peptone 0.1 G_PER_L
7 Yeast extract 0.1 G_PER_L
8 Glucose
C6H12O6 180.156 g/mol
0.1 G_PER_L CHEBI:17234
glucose
9 Agar 18 G_PER_L CHEBI:2509
agar
10 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
10 G_PER_L CHEBI:44557
nitrilotriacetic acid
11 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
0.00925 G_PER_L CHEBI:91249
ammonium molybdate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.599 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.099, 0.5]
13 Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
14 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.095 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
15 MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
0.154 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.0392 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.0248 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
18 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0177 G_PER_L CHEBI:131366
disodium tetraborate decahydrate

Preparation

  1. Adjust pH to 5.8 - 6.5.
  2. Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
  3. Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:14.821073Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1557
2026-03-13T03:09:21.435965Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:00.602708Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:001034
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.234926+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PALUDISPHAERA BOREALIS MEDIUM (PX4)" to "paludisphaera_borealis_medium_px4" for consistent matching
2026-05-20T06:02:01.805038+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5.8-6.5' -> {'min': 5.8, 'max': 6.5}
2026-06-05T07:41:40.840311+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.864124+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:52.220762+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a0dc991be4d504aab33a4faa3d9c95d45763bc5b36eef128966bac27357731f9