planctomyces_medium_with_double_concentrated_artifical_sea_water (CultureMech:001037)

Overview

External ontology

mediadive.medium:1560a (source link unavailable) — PLANCTOMYCES MEDIUM WITH DOUBLE CONCENTRATED ARTIFICAL SEA WATER

Applications

Microbial cultivation

Synonyms

{'name': 'marines_planctomyces_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1560', 'original_category': 'specialized'}
{'name': 'marines_planctomyces_medium_modified_i', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1695', 'original_category': 'specialized'}

pH

7.5

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1560a.pdf

Composition

flowchart LR
    CultureMech_001037["planctomyces_medium_with_double_concentrated_artifical_sea_water"]
    ing0_Peptone["Peptone"]
    CultureMech_001037 --> ing0_Peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_001037 --> ing1_Yeast_extract
    ing2_HEPES["HEPES"]
    CultureMech_001037 --> ing2_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing2_HEPES -.-> CHEBI_46756
    ing3_Agar["Agar"]
    CultureMech_001037 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Double_distilled_water["Double distilled water"]
    CultureMech_001037 --> ing4_Double_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Double_distilled_water -.-> CHEBI_15377
    ing5_N_Acetylglucosamine["N-Acetylglucosamine"]
    CultureMech_001037 --> ing5_N_Acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing5_N_Acetylglucosamine -.-> CHEBI_59640
    ing6_Glucose["Glucose"]
    CultureMech_001037 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_NaCl["NaCl"]
    CultureMech_001037 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Na2SO4["Na2SO4"]
    CultureMech_001037 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_001037 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_CaCl2["CaCl2"]
    CultureMech_001037 --> ing10_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing10_CaCl2 -.-> CHEBI_3312
    ing11_NaHCO3["NaHCO3"]
    CultureMech_001037 --> ing11_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing11_NaHCO3 -.-> CHEBI_32139
    ing12_KCl["KCl"]
    CultureMech_001037 --> ing12_KCl
    CHEBI_32588["CHEBI:32588"]
    ing12_KCl -.-> CHEBI_32588
    ing13_KBr["KBr"]
    CultureMech_001037 --> ing13_KBr
    CHEBI_32030["CHEBI:32030"]
    ing13_KBr -.-> CHEBI_32030
    ing14_H3BO3["H3BO3"]
    CultureMech_001037 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_SrCl2["SrCl2"]
    CultureMech_001037 --> ing15_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing15_SrCl2 -.-> CHEBI_36383
    ing16_NaF["NaF"]
    CultureMech_001037 --> ing16_NaF
    CHEBI_28741["CHEBI:28741"]
    ing16_NaF -.-> CHEBI_28741
    ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_001037 --> ing17_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing18_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_001037 --> ing18_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing18_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing19_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_001037 --> ing19_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing19_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing20_Na2MoO7O4_x_2_H2O["Na2MoO7O4 x 2 H2O"]
    CultureMech_001037 --> ing20_Na2MoO7O4_x_2_H2O
    ing21_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_001037 --> ing21_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing21_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing22_Na_EDTA["Na-EDTA"]
    CultureMech_001037 --> ing22_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing22_Na_EDTA -.-> CHEBI_64734
    ing23_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_001037 --> ing23_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing23_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing24_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_001037 --> ing24_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing24_MnSO4_x_H2O -.-> CHEBI_86364
    ing25_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_001037 --> ing25_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing25_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing26_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_001037 --> ing26_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing26_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing27_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_001037 --> ing27_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing27_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing28_Na_Nitrilotriacetat["Na-Nitrilotriacetat"]
    CultureMech_001037 --> ing28_Na_Nitrilotriacetat
    ing29_Fe_SO4_x_7_H2O["Fe SO4 x 7 H2O"]
    CultureMech_001037 --> ing29_Fe_SO4_x_7_H2O
    ing29_Fe_SO4_x_7_H2O -.-> CHEBI_75836
    CultureMech_001037 --> more["...15 more"]

Ingredients (45)

#	Ingredient	Concentration	Ontology	Notes
1	Peptone	0.25 G_PER_L
2	Yeast extract	0.25 G_PER_L
3	HEPES	2.38 G_PER_L	CHEBI:46756 HEPES
4	Agar	15 G_PER_L	CHEBI:2509 agar
5	Double distilled water H2O 18.015 g/mol	3730.0 G_PER_L	CHEBI:15377 water	[Merged 4 duplicates: 730.0, 1000.0, 1000.0, 1000.0]
6	N-Acetylglucosamine	40 G_PER_L	CHEBI:59640 N-acetylglucosamine
7	Glucose C6H12O6 180.156 g/mol	0.25 G_PER_L	CHEBI:17234 glucose
8	NaCl Cl.Na 58.443 g/mol	93.88 G_PER_L	CHEBI:26710 sodium chloride
9	Na2SO4 2Na.O4S 142.043 g/mol	15.68 G_PER_L	CHEBI:32149 sodium sulfate
10	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	42.56 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
11	CaCl2 Ca.2Cl 110.984 g/mol	5.72 G_PER_L	CHEBI:3312 calcium dichloride
12	NaHCO3 CHO3.Na 84.006 g/mol	0.76 G_PER_L	CHEBI:32139 sodium hydrogencarbonate
13	KCl Cl.K 74.551 g/mol	2.76 G_PER_L	CHEBI:32588 potassium chloride
14	KBr Br.K 119.002 g/mol	0.38 G_PER_L	CHEBI:32030 potassium bromide
15	H3BO3 H3BO3 61.833 g/mol	0.11 G_PER_L	CHEBI:33118 boric acid	[Merged 2 duplicates: 0.1, 0.01]
16	SrCl2 2Cl.Sr 158.526 g/mol	0.16 G_PER_L	CHEBI:36383 strontium dichloride
17	NaF F.Na 41.988 g/mol	0.012 G_PER_L	CHEBI:28741 sodium fluoride
18	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	10 G_PER_L	CHEBI:44557 nitrilotriacetic acid
19	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	29.7 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
20	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	3.34 G_PER_L	CHEBI:86158 calcium chloride dihydrate
21	Na2MoO7O4 x 2 H2O	0.01267 G_PER_L
22	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.599 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	[Merged 2 duplicates: 0.099, 0.5]
23	Na-EDTA C10H14N2O8.2Na 336.208 g/mol	0.25 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)
24	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	1.095 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate
25	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.654 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	[Merged 2 duplicates: 0.154, 0.5]
26	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.0492 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	[Merged 2 duplicates: 0.0392, 0.01]
27	Co(NO3)2 x 6 H2O Co.6H2O.2NO3 291.031 g/mol	0.12480000000000001 G_PER_L	CHEBI:86214 cobalt dinitrate hexahydrate	[Merged 2 duplicates: 0.0248, 0.1]
28	Na2B4O7 x 10 H2O 10H2O.H4B4O9.2Na 417.401 g/mol	0.0177 G_PER_L	CHEBI:131366 disodium tetraborate decahydrate
29	Na-Nitrilotriacetat	1.5 G_PER_L
30	Fe SO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.1 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
31	ZnCl2 2Cl.Zn 136.296 g/mol	0.1 G_PER_L	CHEBI:49976 zinc dichloride
32	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.05 G_PER_L	CHEBI:34887 nickel dichloride
33	H2SeO3 H2O3Se 128.973 g/mol	0.05 G_PER_L	CHEBI:26642 selenous acid
34	AlK(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.01 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate
35	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.01 G_PER_L	CHEBI:75213 sodium molybdate dihydrate
36	Na2WO4 x 2 H2O 2H2O.2Na.O4W 329.846 g/mol	0.01 G_PER_L	CHEBI:63939 sodium tungstate dihydrate
37	Biotin C10H16N2O3S 244.316 g/mol	0.004 G_PER_L	CHEBI:15956 biotin
38	Folic acid C19H19N7O6 441.404 g/mol	0.004 G_PER_L	CHEBI:27470 folic acid
39	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	0.02 G_PER_L	CHEBI:30961 pyridoxine hydrochloride
40	Riboflavine C17H20N4O6 376.369 g/mol	0.01 G_PER_L	CHEBI:17015 riboflavin
41	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.01 G_PER_L	CHEBI:132751 thiamine hydrochloride dihydrate
42	Nicotinamide C6H6N2O 122.127 g/mol	0.01 G_PER_L	CHEBI:17154 nicotinamide
43	D-Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.01 G_PER_L	CHEBI:31345 Calcium pantothenate
44	Vitamin B12	0.0002 G_PER_L	CHEBI:176843 vitamin B12
45	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.01 G_PER_L	CHEBI:30753 4-aminobenzoic acid

Preparation

Sterilize 20 min./121°C. After cooling to 60°C add:
Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l medium of 6 N KOH).
Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

planctomyces_medium_with_double_concentrated_artifical_sea_water (CultureMech:001037)
marines_planctomyces_medium (CultureMech:015343)
marines_planctomyces_medium_modified_i (CultureMech:015345)

Timestamp	Curator	Action	Notes
2026-01-26T17:53:14.832547Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1560a
2026-01-28T19:48:40.763431Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:40.379061Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 8 duplicate ingredient(s)
2026-03-13T06:19:00.634955Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:001037
2026-03-17T06:30:52.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:12.264921+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "PLANCTOMYCES MEDIUM WITH DOUBLE CONCENTRATED ARTIFICAL SEA WATER" to "planctomyces_medium_with_double_concentrated_artifical_sea_water" for consistent matching
2026-06-05T07:41:42.146591+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 40 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.280783+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:53.191873+00:00	recipe-merger	Merged 3 duplicate recipes into canonical record	Sources: planctomyces_medium_with_double_concentrated_artifical_sea_water, marines_planctomyces_medium, marines_planctomyces_medium_modified_i
2026-08-06T03:05:53.191947+00:00	merge_recipes.py	MERGED_RECIPES	merged 3 source recipe(s) on fingerprint 6c4ba7fb453faf9ea7b760e02330356c40825b6df5b1bdc2d87a8cf579199141