Overview
- External ontology
- mediadive.medium:1565 (source link unavailable) — ALKALIPHILUS TRANSVAALENSIS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 9.8, 'max': 10.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1565.pdf
Composition
flowchart LR
CultureMech_001041["alkaliphilus_transvaalensis_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_001041 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_001041 --> ing1_Tryptone
ing2_Na2_tartrate_x_2_H2O["Na2-tartrate x 2 H2O"]
CultureMech_001041 --> ing2_Na2_tartrate_x_2_H2O
ing3_Na3_citrate["Na3-citrate"]
CultureMech_001041 --> ing3_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing3_Na3_citrate -.-> CHEBI_53258
ing4_NaNO3["NaNO3"]
CultureMech_001041 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_001041 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Ferric_citrate_monohydrate["Ferric citrate monohydrate"]
CultureMech_001041 --> ing6_Ferric_citrate_monohydrate
CHEBI_144434["CHEBI:144434"]
ing6_Ferric_citrate_monohydrate -.-> CHEBI_144434
ing7_Na2SO4["Na2SO4"]
CultureMech_001041 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_Na2CO3["Na2CO3"]
CultureMech_001041 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_NaHCO3["NaHCO3"]
CultureMech_001041 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_001041 --> ing10_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing11_NaCl["NaCl"]
CultureMech_001041 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_K2HPO4["K2HPO4"]
CultureMech_001041 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_001041 --> ing13_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_001041 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_001041 --> ing15_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing15_MgCl2_x_6_H2O -.-> CHEBI_86345
ing16_KCl["KCl"]
CultureMech_001041 --> ing16_KCl
CHEBI_32588["CHEBI:32588"]
ing16_KCl -.-> CHEBI_32588
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_001041 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_001041 --> ing18_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing19_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_001041 --> ing19_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing19_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing20_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_001041 --> ing20_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing20_Nitrilotriacetic_acid -.-> CHEBI_44557
ing21_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_001041 --> ing21_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing21_MnSO4_x_H2O -.-> CHEBI_86364
ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_001041 --> ing22_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
ing23_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_001041 --> ing23_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing23_CoSO4_x_7_H2O -.-> CHEBI_91244
ing24_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_001041 --> ing24_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing24_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing25_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_001041 --> ing25_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing25_CuSO4_x_5_H2O -.-> CHEBI_31440
ing26_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_001041 --> ing26_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing26_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing27_H3BO3["H3BO3"]
CultureMech_001041 --> ing27_H3BO3
CHEBI_33118["CHEBI:33118"]
ing27_H3BO3 -.-> CHEBI_33118
ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_001041 --> ing28_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_001041 --> ing29_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Tryptone | 2 G_PER_L | ||
| 3 | Na2-tartrate x 2 H2O | 1 G_PER_L | ||
| 4 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
1 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.25 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.25 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
Ferric citrate monohydrate
C6H5O7.Fe.H2O 262.959 g/mol
|
0.2 G_PER_L |
CHEBI:144434
iron(III) citrate monohydrate
|
|
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.25 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
30.703 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 29.703, 1.0] |
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 14 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.238614 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.138614, 0.1] |
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.36634, 3.0] |
| 16 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13861 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 17 |
KCl
Cl.K 74.551 g/mol
|
0.326733 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03049505 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.00049505, 0.03] |
| 19 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00079505 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
[Merged 2 duplicates: 0.00049505, 0.0003] |
| 20 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00990099 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 21 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 22 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 23 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 24 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 25 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 26 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 27 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 28 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 29 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 30 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Dissolve ingredients except carbonate, bicarbonate, and thiosulfate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and bicarbonate, adjust pH to 10 with KOH, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add thiosulfate to the autoclaved medium from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. Adjust the pH of the complete medium to 9.8 - 10.0.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- alkaliphilus_transvaalensis_medium (CultureMech:001041)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:14.851332Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1565 |
| 2026-01-28T19:48:14.740309Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:52.886441Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:00.670266Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:001041 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:12.302860+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALIPHILUS TRANSVAALENSIS MEDIUM" to "alkaliphilus_transvaalensis_medium" for consistent matching |
| 2026-05-20T06:01:08.054416+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '9.8-10' -> {'min': 9.8, 'max': 10.0} |
| 2026-06-05T07:40:48.200827+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.592040+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:46:52.911598+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.676305+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5e8d8489fa043efc70220e52c2c11af1d339c291e574f90636c2c40ab1cfb763 |